Linked Life Data

19292490

Source:http://linkedlifedata.com/resource/pubmed/id/19292490

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2009-3-18
pubmed:abstractText
DNA strands containing an unnatural T-triazole-T linkage have been synthesized by click DNA ligation between oligonucleotides with 3'-AZT and 5'-propargylamido dT and amplified efficiently by polymerase chain reaction (PCR) using several different polymerases. DNA sequencing of PCR amplicons and clones in two different sequence contexts revealed the presence of a single thymidine at the ligation site. The remarkable ability of thermostable polymerases to reproducibly copy DNA templates containing such an unnatural backbone opens up intriguing possibilities in gene synthesis, genetic analysis, biology, and nanotechnology.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1520-5126
pubmed:author
pubmed:issnType
Electronic
pubmed:day
25
pubmed:volume
131
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3958-64
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Synthesis and polymerase chain reaction amplification of DNA strands containing an unnatural triazole linkage.
pubmed:affiliation
School of Chemistry, University of Southampton, Highfield, Southampton, SO17 1BJ, United Kingdom.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't