Linked Life Data

19289032

Source:http://linkedlifedata.com/resource/pubmed/id/19289032

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2009-3-17
pubmed:abstractText
Characterization of the mechanisms by which proteins fold into their native conformations is important not only for protein structure prediction and design but also because protein misfolding intermediates may play critical roles in fibril formation that are commonplace in neurodegenerative disorders. In practice, the study of folding pathways is complicated by the fact that for the most part intermediates are low-populated and short-lived so that biophysical studies are difficult. Due to recent methodological advances, relaxation dispersion NMR spectroscopy has emerged as a particularly powerful tool to obtain high-resolution structural information about protein folding events on the millisecond timescale. Applications of the methodology to study the folding of SH3 domains have shown that folding proceeds via previously undetected on-pathway intermediates, sometimes stabilized by nonnative long-range interactions. The relaxation dispersion approach provides a detailed kinetic and thermodynamic description of the folding process as well as the promise of obtaining an atomic level structural description of intermediate states. We review the concerted application of a variety of recently developed NMR relaxation dispersion experiments to obtain a "high-resolution" picture of the folding pathway of the A39V/N53P/V55L Fyn SH3 domain.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-10542091, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-10542092, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-11460554, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-11462813, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-11786916, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-11887182, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-1201909, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-12829464, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-14516751, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-14580191, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-15282609, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-15283915, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-15283916, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-15303831, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-15469926, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-1569556, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-15808232, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-15935381, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-16222301, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-16262426, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-16604422, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-16683753, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-16683757, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-16683758, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-16922492, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-16989862, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-17109883, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-17239580, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-17535901, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-17592113, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-17689561, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-17898173, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-18275162, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-18326625, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-18405419, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-18574698, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-18626019, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-18701719, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-18848567, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-19152327, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-1931967, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-2009257, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-4124164, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-7687536, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-8535251, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-9023341, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-9485402, http://linkedlifedata.com/resource/pubmed/commentcorrection/19289032-9519300
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1542-0086
pubmed:author
pubmed:issnType
Electronic
pubmed:day
18
pubmed:volume
96
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2045-54
pubmed:dateRevised
2010-9-23
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Relaxation dispersion NMR spectroscopy as a tool for detailed studies of protein folding.
pubmed:affiliation
Department of Molecular Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada.
pubmed:publicationType
Journal Article, Review, Research Support, Non-U.S. Gov't