Source:http://linkedlifedata.com/resource/pubmed/id/19288186
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
2009-6-15
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pubmed:abstractText |
Immunohistochemistry for neuron-specific nuclear protein (NeuN), caspase-3, calcitonin gene-related peptide (CGRP), and calcium-binding proteins was performed on the trigeminal ganglion (TG) in wild type and Brn-3a knockout mice at embryonic days 12.5-16.5 (E12.5-E16.5). In Brn-3a knockout mice, the number of NeuN-immunoreactive (ir) neuron profiles increased at E14.5 (40.0% increase) and decreased at E16.5 (28.3% reduction) compared to wild type mice. Caspase-3-ir neuron profiles were abundant in the TG of wild type mice at E12.5-E16.5. However, the loss of Brn-3a decreased the number of caspase-3-ir neuron profiles at E12.5 (69.7% reduction) and E14.5 (51.7% reduction). At E16.5, the distribution of caspase-3-ir neuron profiles was barely affected by the deficiency. CGRP-ir neuron profiles were observed in the TG of wild type mice but not knockout mice at E12.5. At E14.5 and E16.5, CGRP-ir neuron profiles were abundant in both wild type and knockout mice. Calbindin D-28 k (CB)-ir neuron profiles decreased in the TG of mutant mice at E12.5 compared to wild type mice (56.4% reduction). At E14.5, however, Brn-3a deficiency transiently increased CB-ir neuron profiles (169.4% increase as compared to wild type mice). Calretinin (CR)-ir neuron profiles could not be detected in the TG of wild type mice at E12.5-16.5. However, numerous CR-ir neuron profiles transiently appeared in the knockout mouse at E14.5. Parvalbumin (PV)-ir neurons appeared in wild type and knockout mice at E14.5. At this stage, the number of large (>50 mum(2)) PV-ir neuron profiles in knockout mice was fewer than that in wild type mice. The number and cell size of PV-ir neuron profiles were barely affected by the deficiency at E16.5. The present study indicates that the loss of Brn-3a causes increase of TG neurons at E14.5 and decrease of TG neurons at E16.5. It is also suggested that Brn-3a deficiency affects the number and cell size of CGRP- and calcium-binding protein-containing neurons at E12.5 and E14.5. Caspase-3-dependent cell death of CB- and CR-ir neurons may be suppressed by the deficiency at E14.5.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcitonin Gene-Related Peptide,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Binding Protein, Vitamin...,
http://linkedlifedata.com/resource/pubmed/chemical/Caspase 3,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/NeuN protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Parvalbumins,
http://linkedlifedata.com/resource/pubmed/chemical/Pou4f1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor Brn-3A,
http://linkedlifedata.com/resource/pubmed/chemical/calbindin,
http://linkedlifedata.com/resource/pubmed/chemical/calretinin
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pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
1573-6830
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
691-8
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pubmed:meshHeading |
pubmed-meshheading:19288186-Animals,
pubmed-meshheading:19288186-Calcitonin Gene-Related Peptide,
pubmed-meshheading:19288186-Calcium-Binding Protein, Vitamin D-Dependent,
pubmed-meshheading:19288186-Caspase 3,
pubmed-meshheading:19288186-Cell Count,
pubmed-meshheading:19288186-Embryonic Development,
pubmed-meshheading:19288186-Immunohistochemistry,
pubmed-meshheading:19288186-Mice,
pubmed-meshheading:19288186-Molecular Weight,
pubmed-meshheading:19288186-Nerve Tissue Proteins,
pubmed-meshheading:19288186-Neurons,
pubmed-meshheading:19288186-Nuclear Proteins,
pubmed-meshheading:19288186-Parvalbumins,
pubmed-meshheading:19288186-Transcription Factor Brn-3A,
pubmed-meshheading:19288186-Trigeminal Ganglion
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pubmed:year |
2009
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pubmed:articleTitle |
Brn-3a deficiency transiently increases expression of calbindin D-28 k and calretinin in the trigeminal ganglion during embryonic development.
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pubmed:affiliation |
Department of Oral Function and Anatomy, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama, 700-8525, Japan. hiroichi@md.okayama-u.ac.jp
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pubmed:publicationType |
Journal Article
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