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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
2009-3-19
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pubmed:databankReference |
http://linkedlifedata.com/resource/pubmed/xref/PubChem-Substance/57244616,
http://linkedlifedata.com/resource/pubmed/xref/PubChem-Substance/57244617,
http://linkedlifedata.com/resource/pubmed/xref/PubChem-Substance/57244618,
http://linkedlifedata.com/resource/pubmed/xref/PubChem-Substance/57244619,
http://linkedlifedata.com/resource/pubmed/xref/PubChem-Substance/57244620,
http://linkedlifedata.com/resource/pubmed/xref/PubChem-Substance/57244621,
http://linkedlifedata.com/resource/pubmed/xref/PubChem-Substance/57244622,
http://linkedlifedata.com/resource/pubmed/xref/PubChem-Substance/57244623,
http://linkedlifedata.com/resource/pubmed/xref/PubChem-Substance/57244624,
http://linkedlifedata.com/resource/pubmed/xref/PubChem-Substance/57244625,
http://linkedlifedata.com/resource/pubmed/xref/PubChem-Substance/57244626,
http://linkedlifedata.com/resource/pubmed/xref/PubChem-Substance/57244627,
http://linkedlifedata.com/resource/pubmed/xref/PubChem-Substance/57244628,
http://linkedlifedata.com/resource/pubmed/xref/PubChem-Substance/57244629,
http://linkedlifedata.com/resource/pubmed/xref/PubChem-Substance/57244630,
http://linkedlifedata.com/resource/pubmed/xref/PubChem-Substance/57244631,
http://linkedlifedata.com/resource/pubmed/xref/PubChem-Substance/57244632,
http://linkedlifedata.com/resource/pubmed/xref/PubChem-Substance/57244633,
http://linkedlifedata.com/resource/pubmed/xref/PubChem-Substance/57244634
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pubmed:abstractText |
Stepwise differentiation from embryonic stem cells (ESCs) to functional insulin-secreting beta cells will identify key steps in beta-cell development and may yet prove useful for transplantation therapy for diabetics. An essential step in this schema is the generation of pancreatic progenitors--cells that express Pdx1 and produce all the cell types of the pancreas. High-content chemical screening identified a small molecule, (-)-indolactam V, that induces differentiation of a substantial number of Pdx1-expressing cells from human ESCs. The Pdx1-expressing cells express other pancreatic markers and contribute to endocrine, exocrine and duct cells, in vitro and in vivo. Further analyses showed that (-)-indolactam V works specifically at one stage of pancreatic development, inducing pancreatic progenitors from definitive endoderm. This study describes a chemical screening platform to investigate human ESC differentiation and demonstrates the generation of a cell population that is a key milepost on the path to making beta cells.
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pubmed:grant |
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1552-4469
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pubmed:author |
pubmed-author:BorowiakMalgorzataM,
pubmed-author:ChenShuibingS,
pubmed-author:DavidowLanceL,
pubmed-author:FoxJulia LJL,
pubmed-author:LamKelvinK,
pubmed-author:MaehrRenéR,
pubmed-author:MeltonDouglasD,
pubmed-author:OsafuneKenjiK,
pubmed-author:PengLee FLF,
pubmed-author:RubinLee LLL,
pubmed-author:SchreiberStuart LSL
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pubmed:issnType |
Electronic
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
258-65
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pubmed:dateRevised |
2011-6-1
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pubmed:meshHeading |
pubmed-meshheading:19287398-Animals,
pubmed-meshheading:19287398-Biological Markers,
pubmed-meshheading:19287398-Carcinogens,
pubmed-meshheading:19287398-Cell Differentiation,
pubmed-meshheading:19287398-Cell Line,
pubmed-meshheading:19287398-Embryonic Stem Cells,
pubmed-meshheading:19287398-Homeodomain Proteins,
pubmed-meshheading:19287398-Humans,
pubmed-meshheading:19287398-Indoles,
pubmed-meshheading:19287398-Insulin-Secreting Cells,
pubmed-meshheading:19287398-Lactams,
pubmed-meshheading:19287398-Mice,
pubmed-meshheading:19287398-Trans-Activators
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pubmed:year |
2009
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pubmed:articleTitle |
A small molecule that directs differentiation of human ESCs into the pancreatic lineage.
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pubmed:affiliation |
Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Cambridge, Massachusetts, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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