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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
2010-11-24
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pubmed:abstractText |
The hippocampus is insensitive to strong inflammatory stimulus under normal conditions and one of the most severely affected areas in Alzheimer's disease. We have analyzed the effect of chronic stress for 9 days in the hippocampus unilaterally injected with LPS. In non-stressed rats, LPS injection failed to activate microglia although a subset of degenerating cells in the CA1 area was evident. This effect was not accompanied by loss of Neu-N positive neurons in the CA1 area. In stressed rats, LPS injection had a dramatic effect in activating microglia along with astrogliosis and BDNF mRNA induction. NeuN immunostaining demonstrated a loss of about 50% of CA1 pyramidal neurons under these conditions. Fluoro jade B histochemistry demonstrated the presence of degenerating cells in most of CA1 area. Mechanistically, combination of chronic stress and LPS resulted in prominent activation of MAPKs including JNK, p38 and ERK clearly different from LPS injection in controls. Further, LPS+stress induced a dramatic decrease in phosphorylated levels of both Akt and CREB, which fully supports a consistent deleterious state in the hippocampal system under these conditions. Treatment with RU486, a potent inhibitor of glucocorticoid receptor activation, significantly protected animals against the deleterious effects observed in LPS-stressed animals.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/6-iodo-5-hydroxy-eicosatrienoic...,
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Brain-Derived Neurotrophic Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Corticosterone,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Signal-Regulated MAP...,
http://linkedlifedata.com/resource/pubmed/chemical/Glial Fibrillary Acidic Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Glutamate Decarboxylase,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type I,
http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide Synthase Type II,
http://linkedlifedata.com/resource/pubmed/chemical/Organic Chemicals,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphopyruvate Hydratase,
http://linkedlifedata.com/resource/pubmed/chemical/Progesterone,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha,
http://linkedlifedata.com/resource/pubmed/chemical/fluoro jade,
http://linkedlifedata.com/resource/pubmed/chemical/glutamate decarboxylase 1
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
1558-1497
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pubmed:author |
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pubmed:copyrightInfo |
Copyright © 2009 Elsevier Inc. All rights reserved.
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pubmed:issnType |
Electronic
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pubmed:volume |
32
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
85-102
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pubmed:meshHeading |
pubmed-meshheading:19286276-Animals,
pubmed-meshheading:19286276-Arachidonic Acids,
pubmed-meshheading:19286276-Body Weight,
pubmed-meshheading:19286276-Brain-Derived Neurotrophic Factor,
pubmed-meshheading:19286276-Cell Count,
pubmed-meshheading:19286276-Corticosterone,
pubmed-meshheading:19286276-Disease Models, Animal,
pubmed-meshheading:19286276-Extracellular Signal-Regulated MAP Kinases,
pubmed-meshheading:19286276-Gene Expression Regulation,
pubmed-meshheading:19286276-Glial Fibrillary Acidic Protein,
pubmed-meshheading:19286276-Glutamate Decarboxylase,
pubmed-meshheading:19286276-Hippocampus,
pubmed-meshheading:19286276-Lipopolysaccharides,
pubmed-meshheading:19286276-Male,
pubmed-meshheading:19286276-Microglia,
pubmed-meshheading:19286276-Nitric Oxide Synthase Type I,
pubmed-meshheading:19286276-Nitric Oxide Synthase Type II,
pubmed-meshheading:19286276-Organic Chemicals,
pubmed-meshheading:19286276-Phosphopyruvate Hydratase,
pubmed-meshheading:19286276-Progesterone,
pubmed-meshheading:19286276-RNA, Messenger,
pubmed-meshheading:19286276-Rats,
pubmed-meshheading:19286276-Rats, Wistar,
pubmed-meshheading:19286276-Stress, Psychological,
pubmed-meshheading:19286276-Time Factors,
pubmed-meshheading:19286276-Tumor Necrosis Factor-alpha
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pubmed:year |
2011
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pubmed:articleTitle |
Stress is critical for LPS-induced activation of microglia and damage in the rat hippocampus.
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pubmed:affiliation |
Departamento de Bioquímica, Bromatología, Toxicología y Medicina Legal, Facultad de Farmacia, Universidad de Sevilla, C/Prof. García González, 2, 41012 Sevilla, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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