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pubmed:abstractText |
Characterization of gene expression programs and pathways important for normal and cancer stem cells has become an active area of investigation. Microarray analysis of various cell populations provides an opportunity to assess genomewide expression programs to define cellular identity and to potentially identify pathways activated in various stem cells. Here we describe methods to isolate a leukemia stem cell population, amplify RNA, and perform microarray analyses.
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pubmed:affiliation |
Department of Pediatric Oncology, Division of Hematology/Oncology, Children's Hospital, Dana Farber Cancer Insitute, Harvard Medical School, Boston, MA, USA.
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