Linked Life Data

19274771

Source:http://linkedlifedata.com/resource/pubmed/id/19274771

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2009-4-2
pubmed:abstractText
We investigated the effects of lipids on thymocyte function. The effects of application of cholesterol or docosahexaenoic acid (DHA), a C22, omega-3 (n-3) polyunsaturated fatty acid (PUFA), on viability and intracellular calcium concentrations of acutely isolated mouse thymocytes were investigated using flow cytometry. Cholesterol (100 microM) caused significant cell death after 30-60 min whether or not calcium was present in the medium. Cell death was associated with an elevation of intracellular calcium whether or not calcium was present in the extracellular medium. However, the elevation of calcium concentration was not responsible for the cell death since calcium levels in the presence of ionomycin rose higher without significant cell death. DHA had similar actions but was more potent, causing significant cell death and elevation of calcium concentration within 5 min at 1 microM. In the absence of extracellular calcium 1 microM DHA caused 100% cell death within 15 min. Linolenic acid, a C18 omega-3 fatty acid also caused cytotoxicity at low concentrations whether or not albumin was present, but omega-6 or saturated C22 fatty acids were much less effective. These observations demonstrate that thymocyte viability is very sensitive to acute exposure to low concentrations of omega-3 fatty acids.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1099-0844
pubmed:author
pubmed:copyrightInfo
2009 John Wiley & Sons, Ltd.
pubmed:issnType
Electronic
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
155-61
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Effects of cholesterol and docosahexaenoic acid on cell viability and (Ca(2+))(i) levels in acutely isolated mouse thymocytes.
pubmed:affiliation
Institute for Health and the Environment, University at Albany, Rensselaer, NY, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't