Source:http://linkedlifedata.com/resource/pubmed/id/19271956
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
2009-3-10
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| pubmed:databankReference | |
| pubmed:abstractText |
The Magnaporthe oryzae avirulence gene AvrPiz-t activates immunity in a gene-for-gene fashion to rice mediated by the blast resistance gene Piz-t. To dissect the molecular mechanism underlying their recognition, we initiated the cloning of AvrPiz-t using a map-based cloning strategy. The AvrPiz-t gene was delimited to an approximately 21-kb genomic fragment, in which six genes were predicted. Complementation tests of each of these six candidate genes led to the final identification of AvrPiz-t, which encodes a 108-amino-acid predicted secreted protein with unknown function and no homologues in M. oryzae or in other sequenced fungi. We found that AvrPiz-t is present in the virulent isolate GUY11 but contains a Pot3 insertion at a position 462 bp upstream from the start codon. Complementation tests of AvrPiz-t genes driven by promoters of varying length revealed that a promoter larger than 462 bp is essential to maintain the AvrPiz-t function. These results suggest that a Pot3 insertion in GUY11 might interfere with the proper function of AvrPiz-t. Additionally, we found that AvrPiz-t can suppress the programmed cell death triggered by mouse BAX protein in Nicotiana benthamiana, identifying a mechanism by which AvrPiz-t may contribute virulence of M. oryzae.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
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| pubmed:month |
Apr
|
| pubmed:issn |
0894-0282
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| pubmed:author |
pubmed-author:BaohuaWangW,
pubmed-author:FengQiQ,
pubmed-author:FuY PYP,
pubmed-author:HuYajunY,
pubmed-author:LuGuodongG,
pubmed-author:RanL ALA,
pubmed-author:WangGuoliangG,
pubmed-author:WangZhengyiZ,
pubmed-author:WangZonghuaZ,
pubmed-author:WuJunJ,
pubmed-author:ZhangHongyanH,
pubmed-author:ZhangXingX,
pubmed-author:ZhangZhengguangZ,
pubmed-author:ZhaoQiangQ,
pubmed-author:ZhouBoB
|
| pubmed:issnType |
Print
|
| pubmed:volume |
22
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
411-20
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| pubmed:meshHeading |
pubmed-meshheading:19271956-Chromosomes, Artificial, Bacterial,
pubmed-meshheading:19271956-Cloning, Molecular,
pubmed-meshheading:19271956-DNA, Fungal,
pubmed-meshheading:19271956-Fungal Proteins,
pubmed-meshheading:19271956-Genes, Fungal,
pubmed-meshheading:19271956-Genetic Complementation Test,
pubmed-meshheading:19271956-Magnaporthe,
pubmed-meshheading:19271956-Molecular Sequence Data,
pubmed-meshheading:19271956-Mutagenesis, Insertional,
pubmed-meshheading:19271956-Oryza sativa,
pubmed-meshheading:19271956-Plant Diseases,
pubmed-meshheading:19271956-Plant Proteins,
pubmed-meshheading:19271956-Sequence Analysis, DNA
|
| pubmed:year |
2009
|
| pubmed:articleTitle |
The Magnaporthe oryzae avirulence gene AvrPiz-t encodes a predicted secreted protein that triggers the immunity in rice mediated by the blast resistance gene Piz-t.
|
| pubmed:affiliation |
National Center for Gene Research & Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, China.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|