Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
1977-6-30
|
| pubmed:abstractText |
Thymidine kinase (TK) activity of uninfected and in vitro herpes simplex virus type 2 (HSV-2) infected human fetal lung cells was analyzed. Polyacrylamide gel electrophoresis was carried out, and subsequent incubation of equally divided gel slices in the presence of normal or anti-HSV-2 specific TK serum, together with different substrates, was performed. Small amounts of virus-specific enzyme could be detected using this combination of electrophoresis and antibodies and replacing ATP by CTP in the substrate medium.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:issn |
0300-5526
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
8
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
186-92
|
| pubmed:dateRevised |
2008-11-7
|
| pubmed:meshHeading |
pubmed-meshheading:192698-Adenosine Triphosphate,
pubmed-meshheading:192698-Culture Techniques,
pubmed-meshheading:192698-Cytosine Nucleotides,
pubmed-meshheading:192698-Cytosol,
pubmed-meshheading:192698-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:192698-Humans,
pubmed-meshheading:192698-Immune Sera,
pubmed-meshheading:192698-Mitochondria,
pubmed-meshheading:192698-Simplexvirus,
pubmed-meshheading:192698-Thymidine Kinase
|
| pubmed:year |
1977
|
| pubmed:articleTitle |
A sensitive method for the detection of herpes simplex virus type 2 specific thymidine kinase.
|
| pubmed:publicationType |
Journal Article
|