rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2009-3-30
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pubmed:abstractText |
The neuropeptide pituitary adenylate cyclase-activating polypeptide (PACAP) mediates its physiological functions through activation of PAC1, VPAC1 and VPAC2 receptors, and the ubiquitous Ca(2+)-sensor calmodulin has been implicated in PACAP-induced signaling. The immediate early response gene FOS is a well-known marker of neuronal activation, so we used a human neuroblastoma cell line NB-1 to explore the role of calmodulin in PACAP-induced FOS gene expression. We observed both short-term and prolonged altered PACAP-mediated activation of the FOS gene in the presence of the calmodulin-antagonist W-7. NB-1 cells were shown to express PAC1 and VPAC2 receptors, and immunoprecipitation of both receptors displayed a co-association with calmodulin in the absence of Ca(2+). Our findings indicate a novel mechanism of calmodulin in regulating PACAP signaling by possible interaction with the inactive state of PAC1 and VPAC2 receptors.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/ADCYAP1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Calmodulin,
http://linkedlifedata.com/resource/pubmed/chemical/Pituitary Adenylate...,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fos,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Pituitary Adenylate...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Vasoactive Intestinal...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Vasoactive Intestinal...
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1532-2785
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
43
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
53-61
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pubmed:meshHeading |
pubmed-meshheading:19269029-Calmodulin,
pubmed-meshheading:19269029-Cell Line, Tumor,
pubmed-meshheading:19269029-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:19269029-Humans,
pubmed-meshheading:19269029-Neuroblastoma,
pubmed-meshheading:19269029-Pituitary Adenylate Cyclase-Activating Polypeptide,
pubmed-meshheading:19269029-Protein Binding,
pubmed-meshheading:19269029-Proto-Oncogene Proteins c-fos,
pubmed-meshheading:19269029-Receptors, Pituitary Adenylate Cyclase-Activating...,
pubmed-meshheading:19269029-Receptors, Vasoactive Intestinal Peptide, Type II,
pubmed-meshheading:19269029-Receptors, Vasoactive Intestinal Polypeptide, Type I,
pubmed-meshheading:19269029-Signal Transduction,
pubmed-meshheading:19269029-Time Factors
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pubmed:year |
2009
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pubmed:articleTitle |
Calmodulin interacts with PAC1 and VPAC2 receptors and regulates PACAP-induced FOS expression in human neuroblastoma cells.
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pubmed:affiliation |
Department of Clinical Biochemistry, Bispebjerg Hospital, Faculty of Health Sciences, University of Copenhagen, Copenhagen, Denmark. bf11@bbh.regionh.dk
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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