Linked Life Data

19266168

Source:http://linkedlifedata.com/resource/pubmed/id/19266168

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2009-5-5
pubmed:databankReference
pubmed:abstractText
We employed a comparative genomic approach to understand protein phosphatase 2C (PP2C)-mediated abscisic acid (ABA) signaling in the moss Physcomitrella patens. Ectopic expression of Arabidopsis (Arabidopsis thaliana) abi1-1, a dominant mutant allele of ABI1 encoding a PP2C involved in the negative regulation of ABA signaling, caused ABA insensitivity of P. patens both in gene expression of late embryogenesis abundant (LEA) genes and in ABA-induced protonemal growth inhibition. The transgenic abi1-1 plants showed decreased ABA-induced freezing tolerance, and decreased tolerance to osmotic stress. Analyses of the P. patens genome revealed that only two (PpABI1A and PpABI1B) PP2C genes were related to ABI1. In the ppabi1a null mutants, ABA-induced expression of LEA genes was elevated, and protonemal growth was inhibited with lower ABA concentration compared to the wild type. Moreover, ABA-induced freezing tolerance of the ppabi1a mutants was markedly enhanced. We provide the genetic evidence that PP2C-mediated ABA signaling is evolutionarily conserved between Arabidopsis and P. patens.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1573-5028
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
70
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
327-40
pubmed:dateRevised
2009-11-25
pubmed:meshHeading
pubmed-meshheading:19266168-Abscisic Acid, pubmed-meshheading:19266168-Arabidopsis, pubmed-meshheading:19266168-Arabidopsis Proteins, pubmed-meshheading:19266168-Base Sequence, pubmed-meshheading:19266168-Bryopsida, pubmed-meshheading:19266168-Cloning, Molecular, pubmed-meshheading:19266168-Evolution, Molecular, pubmed-meshheading:19266168-Gene Expression Regulation, Plant, pubmed-meshheading:19266168-Genome, Plant, pubmed-meshheading:19266168-Molecular Sequence Data, pubmed-meshheading:19266168-Mutation, pubmed-meshheading:19266168-Phosphoprotein Phosphatases, pubmed-meshheading:19266168-Phylogeny, pubmed-meshheading:19266168-Plants, Genetically Modified, pubmed-meshheading:19266168-RNA, Plant, pubmed-meshheading:19266168-Signal Transduction, pubmed-meshheading:19266168-Stress, Physiological
pubmed:year
2009
pubmed:articleTitle
Functional analyses of the ABI1-related protein phosphatase type 2C reveal evolutionarily conserved regulation of abscisic acid signaling between Arabidopsis and the moss Physcomitrella patens.
pubmed:affiliation
Department of Bioscience, Tokyo University of Agriculture, Setagaya-ku, Tokyo, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't