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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2009-5-27
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pubmed:abstractText |
It is well established that epidermal growth factor (EGF) induces the cytoskeleton reorganization and cell migration through two major signaling cascades: phospholipase C-gamma1 (PLC-gamma1) and Rho GTPases. However, little is known about the cross talk between PLC-gamma1 and Rho GTPases. Here we showed that PLC-gamma1 forms a complex with Rac1 in response to EGF. This interaction is direct and mediated by PLC-gamma1 Src homology 3 (SH3) domain and Rac1 (106)PNTP(109) motif. This interaction is critical for EGF-induced Rac1 activation in vivo, and PLC-gamma1 SH3 domain is actually a potent and specific Rac1 guanine nucleotide exchange factor in vitro. We have also demonstrated that the interaction between PLC-gamma1 SH3 domain and Rac1 play a significant role in EGF-induced F-actin formation and cell migration. We conclude that PLC-gamma1 and Rac1 coregulate EGF-induced cell cytoskeleton remodeling and cell migration by a direct functional interaction.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1944-9917
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
23
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
901-13
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pubmed:meshHeading |
pubmed-meshheading:19264842-Actins,
pubmed-meshheading:19264842-Amino Acid Motifs,
pubmed-meshheading:19264842-Amino Acid Sequence,
pubmed-meshheading:19264842-Animals,
pubmed-meshheading:19264842-COS Cells,
pubmed-meshheading:19264842-Cell Movement,
pubmed-meshheading:19264842-Cercopithecus aethiops,
pubmed-meshheading:19264842-Cytoskeleton,
pubmed-meshheading:19264842-Enzyme Activation,
pubmed-meshheading:19264842-Epidermal Growth Factor,
pubmed-meshheading:19264842-Guanine Nucleotide Exchange Factors,
pubmed-meshheading:19264842-Molecular Sequence Data,
pubmed-meshheading:19264842-Phospholipase C gamma,
pubmed-meshheading:19264842-Protein Binding,
pubmed-meshheading:19264842-rac1 GTP-Binding Protein,
pubmed-meshheading:19264842-src Homology Domains
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pubmed:year |
2009
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pubmed:articleTitle |
PLC-gamma1 and Rac1 coregulate EGF-induced cytoskeleton remodeling and cell migration.
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pubmed:affiliation |
Department of Cell Biology, University of Alberta, Edmonton, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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