Linked Life Data

19264529

Source:http://linkedlifedata.com/resource/pubmed/id/19264529

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
2009-5-26
pubmed:abstractText
Species identification of the early colonizers on a corpse, such as Calliphoridae and Sarcophagidae, provides useful information for estimating the postmortem interval (PMI). However, the morphology of adult flies and nymphs is very similar among species of the same genus, and for many forensic scientists accurate identification is very difficult within their routine work. We present a simple and time-saving method for identification of forensically important flies by comparing sequenced 304 bp of mitochondrial DNA cytochrome oxidase gene subunit I (mtDNA COI). Eggs and larvae were recovered from the corpse during forensic autopsies and then reared in a growth chamber under controlled conditions. The emerging adults were killed by ethyl acetate and male specimens were observed under a stereomicroscope to identify the species based on the shape of the genitalia. Subsequently, DNA was extracted from identified specimens and sequenced mtDNA COI, resulting in the identification of 8 species of 4 genera of forensically important diptera. Moreover, the molecular biological species identification was useful in forensic practice, and there were cases in which analysis of DNA extracted from puparia supposed to be early colonizers of the corpse provided useful information for estimating the PMI. We also report sequencing of mtDNA COI of the green bottle fly, Lucilia ampullacea, newly collected from a corpse in Iwate prefecture.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1873-4162
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
11 Suppl 1
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
S344-7
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Practical applications of molecular biological species identification of forensically important flies.
pubmed:affiliation
Department of Biology, Iwate Medical University, Center for Liberal Arts and Sciences, 2-1-1 Nishi-Tokuta, Iwate 028-3694, Japan. saigusak@iwate-med.ac.jp
pubmed:publicationType
Journal Article