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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
2009-4-27
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pubmed:abstractText |
Indole-3-acetaldoxime (IAOx) is a key branching point between primary and secondary metabolism. IAOx serves as an intermediate in the biosynthesis of indole glucosinolates (I-GLSs), camalexin and the plant hormone indole-3-acetic acid (IAA). The cytochrome P450s CYP79B2 and CYP79B3 catalyze the conversion of tryptophan to IAOx. CYP83B1 channels IAOx into I-GLS biosynthesis, CYP71A13 channels IAOx into camalexin biosynthesis, whereas the IAOx-metabolizing enzyme in IAA biosynthesis is not known. In this report, we demonstrate controlled production of I-GLSs by introducing an ethanol (EtOH)-inducible CYP79B2 construct into double (cyp79b2 cyp79b3) or triple (cyp79b2 cyp79b3 cyp83b1) mutant lines. We show EtOH-dependent induction of camalexin and identify a number of candidate IAA homeostasis- or defense-related genes by clustered microarray analysis. The transgenic mutant lines are thus promising tools for elucidating the interplay between primary and secondary metabolism.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Arabidopsis Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/CYP83B1 protein, Arabidopsis,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 Enzyme System,
http://linkedlifedata.com/resource/pubmed/chemical/Ethanol,
http://linkedlifedata.com/resource/pubmed/chemical/Glucosinolates,
http://linkedlifedata.com/resource/pubmed/chemical/Indoleacetic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Indoles,
http://linkedlifedata.com/resource/pubmed/chemical/Oximes,
http://linkedlifedata.com/resource/pubmed/chemical/Thiazoles,
http://linkedlifedata.com/resource/pubmed/chemical/camalexin,
http://linkedlifedata.com/resource/pubmed/chemical/cytochrome P-450 79B2 protein...,
http://linkedlifedata.com/resource/pubmed/chemical/cytochrome P-450 CYP79B3, Sinapis...,
http://linkedlifedata.com/resource/pubmed/chemical/indole-3-acetaldoxime,
http://linkedlifedata.com/resource/pubmed/chemical/indoleacetic acid
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1432-2048
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
229
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1209-17
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pubmed:meshHeading |
pubmed-meshheading:19263076-Arabidopsis,
pubmed-meshheading:19263076-Arabidopsis Proteins,
pubmed-meshheading:19263076-Biosynthetic Pathways,
pubmed-meshheading:19263076-Chromatography, Liquid,
pubmed-meshheading:19263076-Cluster Analysis,
pubmed-meshheading:19263076-Cytochrome P-450 Enzyme System,
pubmed-meshheading:19263076-Ethanol,
pubmed-meshheading:19263076-Gene Expression Profiling,
pubmed-meshheading:19263076-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:19263076-Gene Expression Regulation, Plant,
pubmed-meshheading:19263076-Glucosinolates,
pubmed-meshheading:19263076-Indoleacetic Acids,
pubmed-meshheading:19263076-Indoles,
pubmed-meshheading:19263076-Mass Spectrometry,
pubmed-meshheading:19263076-Molecular Structure,
pubmed-meshheading:19263076-Mutation,
pubmed-meshheading:19263076-Oligonucleotide Array Sequence Analysis,
pubmed-meshheading:19263076-Oximes,
pubmed-meshheading:19263076-Plants, Genetically Modified,
pubmed-meshheading:19263076-Thiazoles
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pubmed:year |
2009
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pubmed:articleTitle |
Controlled indole-3-acetaldoxime production through ethanol-induced expression of CYP79B2.
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pubmed:affiliation |
VKR Research Centre Pro-Active Plants, Department of Plant Biology and Biotechnology, University of Copenhagen, 40 Thorvaldsensvej, 1871, Frederiksberg C, Denmark.
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pubmed:publicationType |
Journal Article
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