Source:http://linkedlifedata.com/resource/pubmed/id/19259613
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
2009-4-9
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pubmed:abstractText |
Human myeloma cells from about 10% of cases with multiple myeloma expressed CD33 and had monocytoid morphology with convoluted nuclei, and all these patients had no increase in serum CRP values. In CD33(+) myeloma cells as well as myeloma cell lines, CD33 expression levels were correlated with the increased expression levels of CEBPA (C/EBPalpha). This correlation was confirmed by the finding that transfection with the CEBPA gene induced CD33 expression in a CD33(-) myeloma cell line. As suggested by the lack of an increase in serum CRP values in CD33(+) myelomas, IL-6 down-regulated the expression of CD33 in CD33(+) myeloma cell lines along with the down-regulation of CEBPA gene expression. Cucurbitacin I (STAT3 inhibitor), but not U0126 (MAPK inhibitor), could abolish the effect of IL-6. Furthermore, IL-6 up-regulated the expression of MYC via STAT3 phosphorylation and MYC bound to the promoter region of the CEBPA gene followed by the down-regulation of CEBPA expression. It was confirmed that introduction of shRNA for MYC into a CD33(+) myeloma cell line blocked the IL-6-induced down-regulation of CD33 and CEBPA expression. Therefore, these results indicate that IL-6 can reverse the expression level of CD33 by up-regulating MYC followed by the down-regulation of CEBPA expression.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation...,
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/CD33 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/CEBPA protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-6,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-myc,
http://linkedlifedata.com/resource/pubmed/chemical/STAT3 Transcription Factor,
http://linkedlifedata.com/resource/pubmed/chemical/STAT3 protein, human
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1865-3774
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pubmed:author | |
pubmed:issnType |
Electronic
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pubmed:volume |
89
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
310-8
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pubmed:meshHeading |
pubmed-meshheading:19259613-Antigens, CD,
pubmed-meshheading:19259613-Antigens, Differentiation, Myelomonocytic,
pubmed-meshheading:19259613-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:19259613-Down-Regulation,
pubmed-meshheading:19259613-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:19259613-Humans,
pubmed-meshheading:19259613-Interleukin-6,
pubmed-meshheading:19259613-Multiple Myeloma,
pubmed-meshheading:19259613-Phosphorylation,
pubmed-meshheading:19259613-Proto-Oncogene Proteins c-myc,
pubmed-meshheading:19259613-STAT3 Transcription Factor,
pubmed-meshheading:19259613-Tumor Cells, Cultured
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pubmed:year |
2009
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pubmed:articleTitle |
IL-6-induced activation of MYC is responsible for the down-regulation of CD33 expression in CD33+ myeloma cells.
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pubmed:affiliation |
Laboratory of Cellular Signal Analysis, Graduate School of Medicine, Yamaguchi University, Ube, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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