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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2009-4-27
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pubmed:databankReference |
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pubmed:abstractText |
The characterization of AmpC-type beta-lactamases was performed in a collection of 32 clinical Pseudomonas aeruginosa isolates with intermediate susceptibility or resistance to imipenem and ceftazidime. Twenty-one out of those 32 isolates overexpressed AmpC beta-lactamase, and the MICs of ceftazidime and imipenem were reduced after cloxacillin addition. Cloning and sequencing identified 10 AmpC beta-lactamase variants. Reduced susceptibility to imipenem, ceftazidime, and cefepime was observed only with recombinant P. aeruginosa strains expressing an AmpC beta-lactamase that had an alanine residue at position 105. The catalytic efficiencies (k(cat)/K(m)) of the AmpC variants possessing this residue were increased against oxyiminocephalosporins and imipenem. In addition, we show here that those AmpC variants constitute a favorable background for the in vitro selection of imipenem-resistant strains. This report identified a novel resistance mechanism that may contribute to imipenem resistance in P. aeruginosa.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19258272-10441119,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19258272-10858348,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19258272-11179650,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19258272-11451693,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19258272-12019116,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19258272-12039884,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19258272-12878494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19258272-14982755,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19258272-14982805,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19258272-15215122,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19258272-15722393,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19258272-15831827,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19258272-15980372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19258272-16251318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19258272-16569851,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19258272-16641429,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19258272-16801449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19258272-17586561,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19258272-17661704,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19258272-17938181,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19258272-18371063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19258272-7574506,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19258272-7890700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19258272-8068328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19258272-8738844,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19258272-9333046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19258272-9449282,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19258272-9671516
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1098-6596
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
53
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1766-71
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pubmed:dateRevised |
2010-9-22
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pubmed:meshHeading |
pubmed-meshheading:19258272-Anti-Bacterial Agents,
pubmed-meshheading:19258272-Bacterial Proteins,
pubmed-meshheading:19258272-Ceftazidime,
pubmed-meshheading:19258272-Cephalosporinase,
pubmed-meshheading:19258272-Humans,
pubmed-meshheading:19258272-Imipenem,
pubmed-meshheading:19258272-Microbial Sensitivity Tests,
pubmed-meshheading:19258272-Molecular Sequence Data,
pubmed-meshheading:19258272-Pseudomonas aeruginosa,
pubmed-meshheading:19258272-Sequence Analysis, DNA,
pubmed-meshheading:19258272-beta-Lactam Resistance,
pubmed-meshheading:19258272-beta-Lactamases,
pubmed-meshheading:19258272-beta-Lactams
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pubmed:year |
2009
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pubmed:articleTitle |
Extended-spectrum cephalosporinases in Pseudomonas aeruginosa.
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pubmed:affiliation |
Service de Bactériologie-Virologie, INSERM U914 Emerging Resistance to Antibiotics, Assistance Publique/Hôpitaux de Paris, Faculté de Médecine and Université Paris-Sud, Hôpital de Bicêtre, 94275 K.-Bicêtre, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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