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rdf:type |
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|
lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1991-10-29
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pubmed:abstractText |
An enzyme immunoassay was developed to detect anti-Ro(SS-A) autoantibodies. Both Ro-antigen components (52 and 60 kD) were purified from a pig spleen extract, using fast protein liquid chromatography (FPLC). Anti-La, anti-RNP, anti-DNA and anti-Sm antibodies do not react to the purified antigen. There was a strong correlation between anti-Ro activity in EIA and the titers in counter immunoelectrophoresis (rs = 0.893). Anti-Ro antibodies were found in 54 (69.2%) of 78 SLE sera by the developed EIA.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
|
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pubmed:citationSubset |
IM
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pubmed:chemical |
|
|
pubmed:status |
MEDLINE
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pubmed:issn |
0300-9742
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pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:volume |
20
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pubmed:owner |
NLM
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|
pubmed:authorsComplete |
Y
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pubmed:pagination |
267-73
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pubmed:dateRevised |
2007-5-11
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pubmed:meshHeading |
pubmed-meshheading:1925414-Animals,
pubmed-meshheading:1925414-Autoantibodies,
pubmed-meshheading:1925414-Autoantigens,
pubmed-meshheading:1925414-Chromatography, Liquid,
pubmed-meshheading:1925414-Counterimmunoelectrophoresis,
pubmed-meshheading:1925414-Immunoblotting,
pubmed-meshheading:1925414-Immunoenzyme Techniques,
pubmed-meshheading:1925414-Lupus Erythematosus, Systemic,
pubmed-meshheading:1925414-Molecular Weight,
pubmed-meshheading:1925414-RNA, Small Cytoplasmic,
pubmed-meshheading:1925414-Reference Values,
pubmed-meshheading:1925414-Ribonucleoproteins,
pubmed-meshheading:1925414-Swine
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pubmed:year |
1991
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pubmed:articleTitle |
Development of a solid phase enzyme immunoassay for the detection of anti-Ro autoantibodies.
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|
pubmed:affiliation |
Department of Internal Medicine, Medical School (Charité), Humboldt University Berlin, Germany.
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pubmed:publicationType |
Journal Article,
Comparative Study
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