Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
18
pubmed:dateCreated
2009-4-27
pubmed:abstractText
SLAT (SWAP-70-like adaptor protein of T cells) is an adaptor protein expressed in cells of the hematopoietic system. SLAT interacts with and alters the function of small GTPase Rac1 in fibroblasts. In these nonhematopoietic models, the SLAT-Rac interaction leads to changes in F-actin and causes cytoskeletal reorganization. In T cells, SLAT expression regulates the development of T helper cells through Cdc42- and Rac1-mediated activation of the NF-AT transcription factor. Here we show that SLAT is expressed in macrophages. Overexpression of SLAT in a macrophage cell line inhibits the IgG Fcgamma receptor-mediated phagocytic ability of THP1 cells. In bone marrow-derived macrophages, SLAT protein is recruited to the early phagosomes formed via Fcgamma receptor engagement. SLAT recruitment to the phagosome was most efficient when the macrophages express at least one isoform of Rac (Rac1 or Rac2), because SLAT recruitment was reduced in macrophages of Rac-deficient mice. Macrophages derived from animals lacking SLAT show an elevation in the rate of Fcgamma receptor-mediated phagocytosis. The absence of SLAT is associated with an increase in the amount of F-actin formed around these phagosomes as well as an increase in the amount of Rac1 protein recruited to the phagosome. Our results suggest that SLAT acts as a gatekeeper for the amount of Rac recruited to the phagosomes formed by Fcgamma receptor engagement and thus is able to regulate F-actin re-organization and consequently phagocytosis.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-10072071, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-10224076, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-10460589, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-10487749, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-10621974, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-10677525, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-10781611, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-10934035, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-10953003, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-11145705, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-11258418, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-11441091, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-11595183, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-11739662, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-11854399, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-11961559, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-12134158, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-12648457, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-12651066, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-12759446, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-12821647, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-12923183, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-14996945, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-15023524, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-15169870, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-15261675, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-15292195, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-15308574, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-15870290, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-15972681, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-16050141, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-16413920, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-16470246, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-16543474, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-16546099, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-16985500, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-17056570, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-17121847, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-17308335, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-17312158, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-17332738, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-17657315, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-17890224, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-18490410, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-18976935, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-8195699, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-8625410, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-8993005, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-9314552, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-9348306, http://linkedlifedata.com/resource/pubmed/commentcorrection/19251698-9880492
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Actins, http://linkedlifedata.com/resource/pubmed/chemical/DEF6 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Guanine Nucleotide Exchange Factors, http://linkedlifedata.com/resource/pubmed/chemical/NFATC Transcription Factors, http://linkedlifedata.com/resource/pubmed/chemical/Neuropeptides, http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins, http://linkedlifedata.com/resource/pubmed/chemical/RAC1 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Rac1 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, IgG, http://linkedlifedata.com/resource/pubmed/chemical/SLAT protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/cdc42 GTP-Binding Protein, http://linkedlifedata.com/resource/pubmed/chemical/rac GTP-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/rac1 GTP-Binding Protein, http://linkedlifedata.com/resource/pubmed/chemical/rac2 GTP-binding protein
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0021-9258
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
284
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
11882-91
pubmed:dateRevised
2010-9-22
pubmed:meshHeading
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