Linked Life Data

19250807

Source:http://linkedlifedata.com/resource/pubmed/id/19250807

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2009-4-20
pubmed:abstractText
This work investigated the biological influence of water-soluble multiwalled carbon nanotubes (wsMWCNTs) on fibroblast cell growth as a function of concentration control in an aqueous solution. The wsMWCNTs were prepared by an optimal procedure of ultrasonication/concentrated acids oxidation. The concentration of wsMWCNT in the solution was quantified by an established calibration line. A stable concentration of 0.3mg/ml was obtained in the surfactant-free water. The physicochemical properties of wsMWCNTs were characterized using scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), UV/VIS/NIR spectroscopy, and dynamic light scattering (DLS). Cell proliferation and the cell cycle were examined by MTS assay, flow cytometry and TEM respectively. Experimental results showed that the oxidation degree was a key factor that determined the concentration and stability of wsMWCNTs in the aqueous solution. The wsMWCNTs were able to enter into the cells and mainly accumulated in the cytoplasm. The wsMWCNTs-induced variations in cell proliferation and the cell cycle were concentration dependent. Cells cultivated with wsMWCNTs of 0.3mg/ml underwent a dramatic apoptosis. The proliferation was clearly suppressed when the cells were cultivated with wsMWCNTs of 0.03 mg/ml. There were no obvious influences on cell proliferation and the cell cycle when the concentration of wsMWNTs decreased to 0.01 mg/ml.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1873-4367
pubmed:author
pubmed:issnType
Electronic
pubmed:day
1
pubmed:volume
71
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
148-53
pubmed:dateRevised
2009-10-16
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Concentration control of carbon nanotubes in aqueous solution and its influence on the growth behavior of fibroblasts.
pubmed:affiliation
Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100005, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't