Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1977-6-30
pubmed:abstractText
Five continuous mouse mammary tumor cell lines and 12 clonal derivatives have been established from tumors arising in BALB/c or C3H mice either spontaneously or in response to viral (mammary tumor virus), hormonal (17 beta-estradiol), or chemical [7,12-dimethylbenz(alpha)anthracene] stimuli in vivo. The cell lines were examined for the following in vitro growth parameters:plating efficiency, saturation density, population-doubling times, colony formation on plastic surfaces, and anchorage-independent growth in methylcellulose. The majority of the mammary tumor cell lines were transplantable in syngeneic, immunocompetent mice and gave rise to tumors composed of epithelial cells. There was no growth parameter in vitro which invariably correlated with tumorigenicity in vivo. Most of the mammary tumor cell lines appeared to produce C-type virus. Only one, a C3H derivative, appeared to respond to stimulation by glucocorticoids with the enhanced production of B-type virus. Analysis of the growth properties exhibited in culture by transformed mammary epithelial cells revealed marked differences from those previously reported for transformed fibroblasts.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:volume
37
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1892-900
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:192465-Animals, pubmed-meshheading:192465-Cell Adhesion, pubmed-meshheading:192465-Cell Division, pubmed-meshheading:192465-Cell Line, pubmed-meshheading:192465-Cell Transformation, Neoplastic, pubmed-meshheading:192465-Clone Cells, pubmed-meshheading:192465-Epithelial Cells, pubmed-meshheading:192465-Epithelium, pubmed-meshheading:192465-Female, pubmed-meshheading:192465-Hydrocortisone, pubmed-meshheading:192465-Mammary Neoplasms, Experimental, pubmed-meshheading:192465-Mice, pubmed-meshheading:192465-Mice, Inbred BALB C, pubmed-meshheading:192465-Mice, Inbred C3H, pubmed-meshheading:192465-Neoplasm Transplantation, pubmed-meshheading:192465-Oncogenic Viruses, pubmed-meshheading:192465-Retroviridae, pubmed-meshheading:192465-Transformation, Genetic, pubmed-meshheading:192465-Transplantation, Isogeneic
pubmed:year
1977
pubmed:articleTitle
Comparison of the growth properties in vitro and transplantability of continuous mouse mammary tumor cell lines and clonal derivatives.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S.