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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
20
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pubmed:dateCreated |
1991-11-15
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pubmed:abstractText |
The cysteine-rich LIM motif is highly conserved between invertebrates and mammals. This motif shows similarity both to proteins that bind zinc and to ferredoxins, which contain iron-sulfur clusters. Two tandem copies of the LIM motif are found in a number of presumptive transcription factors, including the protein product of the Caenorhabditis elegans cell-lineage gene lin-11. To investigate the possible metal-binding properties of the LIM region of the lin-11 protein, we expressed and purified a 151-amino acid peptide containing the tandem LIM motifs. The purified peptide binds both zinc (two atoms per protein molecule) and iron (as a redox-active iron-sulfur cluster, with four atoms of iron and four atoms of inorganic sulfide per protein molecule). These observations suggest that the LIM motif is a metallodomain that might function in a redox-sensitive regulation of transcription.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924383-14299651,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924383-1672661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924383-1691825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924383-1846973,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924383-1970421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924383-1991317,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924383-2034676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924383-2106626,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924383-2142998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924383-2311586,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924383-2408046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924383-2501659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924383-2548577,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924383-2568852,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924383-2669955,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924383-2726740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924383-2877747,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924383-2898300,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924383-2902928,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924383-3156376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924383-3215510,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924383-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924383-6059350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924383-6094558,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924383-6794614
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
88
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9210-3
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:1924383-Amino Acid Sequence,
pubmed-meshheading:1924383-Animals,
pubmed-meshheading:1924383-Base Sequence,
pubmed-meshheading:1924383-Caenorhabditis,
pubmed-meshheading:1924383-Cloning, Molecular,
pubmed-meshheading:1924383-Iron-Sulfur Proteins,
pubmed-meshheading:1924383-Molecular Sequence Data,
pubmed-meshheading:1924383-Molecular Weight,
pubmed-meshheading:1924383-Oligonucleotides,
pubmed-meshheading:1924383-Peptide Fragments,
pubmed-meshheading:1924383-Plasmids,
pubmed-meshheading:1924383-Polymerase Chain Reaction,
pubmed-meshheading:1924383-Recombinant Proteins,
pubmed-meshheading:1924383-Restriction Mapping,
pubmed-meshheading:1924383-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1924383-Spectrophotometry,
pubmed-meshheading:1924383-Transcription Factors,
pubmed-meshheading:1924383-Zinc Fingers
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pubmed:year |
1991
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pubmed:articleTitle |
The LIM region of a presumptive Caenorhabditis elegans transcription factor is an iron-sulfur- and zinc-containing metallodomain.
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pubmed:affiliation |
Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02215.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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