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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
20
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pubmed:dateCreated |
1991-11-15
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pubmed:abstractText |
dAn.dTn sequences, otherwise known as A tracts, are hotspots for cis-syn thymine dimer formation and deletion mutations induced by UV light. Such A tracts are also known to bend DNA, suggesting that some biological effects of UV light might be related to the distinctive structure and properties of cis-syn dimer-containing A tracts. To investigate the effect of thymine dimer formation on A-tract bending multimers of all possible dimer monoadducts of a dA6.dT6-containing decamer known to bend DNA were prepared along with multimers of a dimer-containing 21-mer of heterogeneous sequence. The characteristic anomalous electrophoretic behavior of the phased A-tract multimers was essentially abolished by dimer formation at the center of the A tract and was only slightly reduced by dimer formation at the ends. These effects are attributed to disruption of the A-tract structure at the site of the dimer, resulting in intact A tracts of reduced length and, hence, reduced bending. This model was suggested by the ability to formulate the estimated bend angles of the dimer-containing A tracts as approximately equal to the sum of the bend angles induced by the dimer and the remaining intact portion of the A tract. Contrary to a previous experimental study that concluded that the thymine dimer bends DNA by approximately 30 degrees, the dimer was determined to bend DNA by only approximately 7 degrees. Reduction of the bending of a DNA sequence by dimer formation may have a number of unpredicted and important biological consequences.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924370-17813621,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924370-2185240,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924370-2197990,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924370-2271562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924370-2315048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924370-2361140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924370-2755998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924370-2789995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924370-2951850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924370-3028790,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924370-3035498,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/1924370-3325999,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/1924370-3479789,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924370-3651409,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924370-3670410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924370-3713816,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924370-3923204,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924370-3960133,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924370-3975615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924370-6096016,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924370-6214637,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/1924370-6739295,
http://linkedlifedata.com/resource/pubmed/commentcorrection/1924370-7045692
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
88
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9072-6
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pubmed:dateRevised |
2010-9-9
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pubmed:meshHeading |
pubmed-meshheading:1924370-Base Composition,
pubmed-meshheading:1924370-Base Sequence,
pubmed-meshheading:1924370-Chromatography, High Pressure Liquid,
pubmed-meshheading:1924370-DNA,
pubmed-meshheading:1924370-DNA Damage,
pubmed-meshheading:1924370-DNA Repair,
pubmed-meshheading:1924370-Molecular Sequence Data,
pubmed-meshheading:1924370-Nucleic Acid Conformation,
pubmed-meshheading:1924370-Oligodeoxyribonucleotides,
pubmed-meshheading:1924370-Pyrimidine Dimers,
pubmed-meshheading:1924370-Structure-Activity Relationship,
pubmed-meshheading:1924370-Ultraviolet Rays
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pubmed:year |
1991
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pubmed:articleTitle |
Site-specific effect of thymine dimer formation on dAn.dTn tract bending and its biological implications.
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pubmed:affiliation |
Department of Chemistry, Washington University, St. Louis, MO 63130-4899.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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