Linked Life Data

19242943

Source:http://linkedlifedata.com/resource/pubmed/id/19242943

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
2009-3-30
pubmed:abstractText
Unique spectral properties of quantum dots (QDs) enable ultrasensitive and long-term biolabeling. Aiming to trace the infection, movement, and localization of viruses in living cells, QD-containing virus-like particles (VLPs) of simian virus 40 (SV40), termed SVLP-QDs, are constructed by in vitro self-assembly of the major capsid protein of SV40. SVLP-QDs show homogeneity in size ( approximately 24 nm), similarity in spectral properties to unencapsidated QDs, and considerable stability. When incubated with living cells, SVLP-QDs are shown to enter the cells by caveolar endocytosis, travel along the microtubules, and accumulate in the endoplasmic reticulum. This process mimics the early infection steps of SV40. This is the first paradigm of imaging viral behaviors with encapsidated QDs in living cells. The method may provide a new alternative for various purposes, such as tracing viruses or viral components, targeted nanoparticle delivery, and probing of drug delivery.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1613-6829
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
718-26
pubmed:dateRevised
2009-5-7
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Imaging viral behavior in Mammalian cells with self-assembled capsid-quantum-dot hybrid particles.
pubmed:affiliation
State Key Laboratory of Virology, Wuhan Institute of Virology Chinese Academy of Sciences No.44, Xiaohongshan, Wuhan 430071, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't