Source:http://linkedlifedata.com/resource/pubmed/id/19242528
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
2009-5-14
|
| pubmed:abstractText |
Adoptive T-cell transfer of in vitro cultured T cells derived from cancer patients with naturally developed immune responses has met with some success as an immunotherapeutic approach, although only a limited number of patients showed spontaneous immune responses. To find alternative ways, such as cancer-specific T-cell receptor (TCR) gene transfer, in preparation for sufficient numbers of antigen-specific T cells is an important issue in the field of adoptive T-cell therapy. Given the inherent disadvantage of alphabeta TCR transfer to other alphabeta T cells, namely the possible formation of mixed TCR heterodimers with endogenous alpha or beta TCR, we employed gammadelta T cells as a target for retroviral transfer of cancer-specific TCR and examined whether gammadelta T cells were useful as an alternative population for TCR transfer. Although retroviral transduction to gammadelta T cells with TCR alphabeta genes alone, isolated from a MAGE-A4(143-151)-specific alphabeta CD8(+) cytotoxic T lymphocyte (CTL) clone, did not provide sufficient affinity to recognize major histocompatibility (MHC)-peptide complexes due to the lack of CD8 co-receptor, gammadelta T cells co-transduced with TCR alphabeta and CD8 alphabeta genes acquired cytotoxicity against tumor cells and produced cytokines in both alphabeta- and gammadelta-TCR-dependent manners. Furthermore, alphabeta TCR and CD8-transduced gammadelta T cells, stimulated either through alphabeta TCR or gammadelta TCR, rapidly responded to target cells compared with conventional alphabeta T cells, reminiscent of gammadelta T cells. We propose alphabeta TCR-transduced gammadelta T cells as an alternative strategy for adoptive T-cell transfer.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
1476-5462
|
| pubmed:author | |
| pubmed:issnType |
Electronic
|
| pubmed:volume |
16
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
620-8
|
| pubmed:meshHeading |
pubmed-meshheading:19242528-Adoptive Transfer,
pubmed-meshheading:19242528-CD8-Positive T-Lymphocytes,
pubmed-meshheading:19242528-Cytotoxicity, Immunologic,
pubmed-meshheading:19242528-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:19242528-Gene Therapy,
pubmed-meshheading:19242528-Genes, Neoplasm,
pubmed-meshheading:19242528-Genetic Vectors,
pubmed-meshheading:19242528-Humans,
pubmed-meshheading:19242528-Lymphocyte Activation,
pubmed-meshheading:19242528-Lymphocyte Transfusion,
pubmed-meshheading:19242528-Neoplasms,
pubmed-meshheading:19242528-Receptors, Antigen, T-Cell, alpha-beta,
pubmed-meshheading:19242528-Receptors, Antigen, T-Cell, gamma-delta,
pubmed-meshheading:19242528-Retroviridae,
pubmed-meshheading:19242528-T-Cell Antigen Receptor Specificity,
pubmed-meshheading:19242528-Transduction, Genetic,
pubmed-meshheading:19242528-Tumor Cells, Cultured
|
| pubmed:year |
2009
|
| pubmed:articleTitle |
Rapid alphabeta TCR-mediated responses in gammadelta T cells transduced with cancer-specific TCR genes.
|
| pubmed:affiliation |
Department of Immunno-Gene Therapy, Mie University Graduate School of Medicine, Mie, Japan.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|