Linked Life Data

19242510

Source:http://linkedlifedata.com/resource/pubmed/id/19242510

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2009-4-16
pubmed:abstractText
Mast cells are widely distributed throughout the body, being preferentially localized at host-environment interfaces. They have long been known as major effector cells in IgE-mediated allergic responses. However, accumulating evidence has provided many new insights into their functions. They are now known to be involved in diverse pathological processes, for example, innate and adaptive immunity. Utility of mast cell-deficient mice and mast cell-knock-in mice has provided powerful models to demonstrate compelling evidence for their in vivo relevance. Conversely, primary cultures of tissue-derived mast cells provide excellent models for in vitro studies of functions at both cellular and molecular levels. Because mast cells exhibit phenotypical and functional heterogeneity in different anatomical sites, it is important to obtain tissue-specific mast cells to clarify their function in tissue. In this regard, researchers have established several methods to prepare mast cells from different tissues, which are technically difficult to obtain at high purity and yield. To overcome these difficulties, we have developed a primary culture system to obtain large numbers of mast cells at high purity from murine fetal skin. In this review, we describe characteristics of such mast cells and their utility in mast cell biology.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
1523-1747
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
129
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1120-5
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Murine fetal skin-derived cultured mast cells: a useful tool for discovering functions of skin mast cells.
pubmed:affiliation
Department of Dermatology, Graduate School of Medicine, Chiba University, Chuo-ku, Chiba, Japan. hmatsue@faculty.chiba-u.jp
pubmed:publicationType
Journal Article, Review, Research Support, Non-U.S. Gov't