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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
2009-3-11
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pubmed:abstractText |
GPR109B (HM74) is a putative G protein-coupled receptor (GPCR) whose cognate ligands have yet to be characterized. GPR109B shows a high degree of sequence similarity to GPR109A, another GPCR that was identified as a high-affinity nicotinic acid (niacin) receptor. However, the affinity of nicotinic acid to GPR109B is very low. In this study, we found that certain aromatic D-amino acids, including D-phenylalanine, D-tryptophan, and the metabolite of the latter, D-kynurenine, decreased the activity of adenylate cyclase in cells transfected with GPR109B cDNA through activation of pertussis toxin (PTX)-sensitive G proteins. These D-amino acids also elicited a transient rise of intracellular Ca(2+) level in cells expressing GPR109B in a PTX-sensitive manner. In contrast, these D-amino acids did not show any effects on cells expressing GPR109A. We found that the GPR109B mRNA is abundantly expressed in human neutrophils. D-phenylalanine and D-tryptophan induced a transient increase of intracellular Ca(2+) level and a reduction of cAMP levels in human neutrophils. Furthermore, knockdown of GPR109B by RNA interference inhibited the D-amino acids-induced decrease of cellular cAMP levels in human neutrophils. These D-amino acids induced chemotactic activity of freshly prepared human neutrophils. We also found that D-phenylalanine and D-tryptophan induced chemotactic responses in Jurkat cells transfected with the GPR109B cDNA but not in mock-transfected Jurkat cells. These results suggest that these aromatic D-amino acids elicit a chemotactic response in human neutrophils via activation of GPR109B.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19237584-10326025,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19237584-11359784,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19237584-11574155,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19237584-12065583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19237584-12522134,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19237584-12563315,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19237584-12646212,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19237584-12711604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19237584-14018705,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19237584-14604536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19237584-15581849,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19237584-15758070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19237584-6105623,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19237584-6300699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19237584-7389911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19237584-7505609,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19237584-7592536,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19237584-7797484,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19237584-9829988
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Amino Acids, Aromatic,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Chemotactic Factors,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/HCAR3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Pertussis Toxin,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, G-Protein-Coupled,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Nicotinic
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1091-6490
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
10
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pubmed:volume |
106
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3930-4
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pubmed:dateRevised |
2011-10-13
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pubmed:meshHeading |
pubmed-meshheading:19237584-Amino Acids, Aromatic,
pubmed-meshheading:19237584-Animals,
pubmed-meshheading:19237584-CHO Cells,
pubmed-meshheading:19237584-Calcium,
pubmed-meshheading:19237584-Chemotactic Factors,
pubmed-meshheading:19237584-Cricetinae,
pubmed-meshheading:19237584-Cricetulus,
pubmed-meshheading:19237584-Cytoplasm,
pubmed-meshheading:19237584-GTP-Binding Proteins,
pubmed-meshheading:19237584-Gene Expression Regulation,
pubmed-meshheading:19237584-Humans,
pubmed-meshheading:19237584-Neutrophils,
pubmed-meshheading:19237584-Pertussis Toxin,
pubmed-meshheading:19237584-RNA, Messenger,
pubmed-meshheading:19237584-Receptors, G-Protein-Coupled,
pubmed-meshheading:19237584-Receptors, Nicotinic
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pubmed:year |
2009
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pubmed:articleTitle |
Aromatic D-amino acids act as chemoattractant factors for human leukocytes through a G protein-coupled receptor, GPR109B.
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pubmed:affiliation |
Yanagisawa Orphan Receptor Project, Exploratory Research for Advanced Technology, Japan Science and Technology Agency, Tokyo 135-0064, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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