Linked Life Data

19237278

Source:http://linkedlifedata.com/resource/pubmed/id/19237278

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2009-3-30
pubmed:abstractText
A simple, rapid and highly sensitive piezoelectric immunosensor has been proposed and applied to detect aflatoxin B(1) (AFB(1)). It is unlikely that direct binding of small molecules such like AFB(1) to the piezoelectric sensor surface could result in a satisfactory detection limit and sensitivity. Thus, indirect competitive immunoassay technique had been used for the detection of the target and gold nanoparticles (GNP) been employed as a 'weight label' to the secondary antibody for amplifying the response. This method is proven in its ability to detect AFB(1) down to a level of 0.01 ng mL(-1) in artificially contaminated milk, which is comparable to or even exceeding the sensitivity of microtitre plate ELISA. Furthermore, the frequency responses of the immunoassay are linearly correlated to the logarithm of AFB(1) concentration in the range of 0.1-100 ng mL(-1). The sensor could be regenerated under very mild conditions simply by immersing the sensor into glycine buffer solution to desorb the combined antibody. It is found that the as-renewed sensor could be reused at least 9 runs without obvious loss of sensing sensitivity.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1873-4235
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2580-5
pubmed:dateRevised
2009-7-14
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Piezoelectric immunosensor with gold nanoparticles enhanced competitive immunoreaction technique for quantification of aflatoxin B1.
pubmed:affiliation
State Key Laboratory of Chemo/Biosensing and Chemometrics, Chemistry and Chemical Engineering College, Hunan University, Changsha 410082, PR China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies