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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1991-11-1
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pubmed:abstractText |
Two murine cell lines that overexpress v-sis/PDGF-2 were used to study the mechanism of cell transformation by SSV (simian sarcoma virus). In contrast to the parental cells that are phenotypically normal and serum-dependent for growth, v-sis-overexpressing cells grow in PDGF-free plasma medium, are unable to enter the G0 state and are highly tumorigenic. Analysis of the expression of some growth factor-induced early response genes in v-sis-overexpressing cells revealed: (a) high and constitutive c-myc mRNA levels in SSV-NRK cells; (b) unaltered levels of fra-1, fos B, jun B and krox 20 transcripts; (c) high and constitutive FOS staining due to c-FOS and FOS-related protein(s); (d) constitutive c-JUN and higher JUN D expression. These results are compatible with a model in which endogenous production of v-sis/PDGF-2 leads to deregulated expression of key cellular transregulators that, in turn, alter the cells' transcriptional program leading to the transformed state and malignancy.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Early Growth Response Protein 2,
http://linkedlifedata.com/resource/pubmed/chemical/Egr2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Oncogene Proteins v-sis,
http://linkedlifedata.com/resource/pubmed/chemical/Platelet-Derived Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Retroviridae Proteins, Oncogenic,
http://linkedlifedata.com/resource/pubmed/chemical/Thymidine,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0950-9232
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
6
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pubmed:geneSymbol |
c-myc,
fos,
fra-1,
jun,
v-sis
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1531-7
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:1923519-3T3 Cells,
pubmed-meshheading:1923519-Animals,
pubmed-meshheading:1923519-Cell Division,
pubmed-meshheading:1923519-Cell Line,
pubmed-meshheading:1923519-Cell Transformation, Neoplastic,
pubmed-meshheading:1923519-DNA Replication,
pubmed-meshheading:1923519-DNA-Binding Proteins,
pubmed-meshheading:1923519-Early Growth Response Protein 2,
pubmed-meshheading:1923519-Gene Expression,
pubmed-meshheading:1923519-Genes, fos,
pubmed-meshheading:1923519-Genes, jun,
pubmed-meshheading:1923519-Genes, myc,
pubmed-meshheading:1923519-Mice,
pubmed-meshheading:1923519-Mice, Inbred BALB C,
pubmed-meshheading:1923519-Oncogene Proteins v-sis,
pubmed-meshheading:1923519-Oncogenes,
pubmed-meshheading:1923519-Platelet-Derived Growth Factor,
pubmed-meshheading:1923519-Protein-Tyrosine Kinases,
pubmed-meshheading:1923519-Retroviridae Proteins, Oncogenic,
pubmed-meshheading:1923519-Thymidine,
pubmed-meshheading:1923519-Transcription, Genetic,
pubmed-meshheading:1923519-Transcription Factors,
pubmed-meshheading:1923519-Transfection,
pubmed-meshheading:1923519-Zinc Fingers
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pubmed:year |
1991
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pubmed:articleTitle |
Imbalanced expression of cellular nuclear oncogenes caused by v-sis/PDGF-2.
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pubmed:affiliation |
Departamento de Bioquímica, Universidade de São Paulo, Brazil.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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