Linked Life Data

19234564

Source:http://linkedlifedata.com/resource/pubmed/id/19234564

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2009-2-23
pubmed:abstractText
The frequency and pattern of irradiation-induced intergenomic chromosome rearrangements were analysed in the mutagenized (M0) and the first selfed (M1) generations of Triticum aestivum L. - Aegilops biuncialis Vis. amphiploids (2n = 70, AABBDDUbUbMbMb) by multicolour genomic in situ hybridization (mcGISH). mcGISH allowed the simultaneous discrimination of individual Ae. biuncialis genomes and wheat chromosomes. Dicentric chromosomes, fragments, and terminal translocations were most frequently induced by gamma-irradiation, but centric fusions and internal exchanges were also more abundant in the treated plants than in control amphiploids. Rearrangements involving the Ub genome (Ub-type aberrations) were more frequent than those involving the Mb genome (Mb-type aberrations). This irradiation sensitivity of the Ub chromosomes was attributed to their centromeric or near-centromeric regions, since Ub-type centric fusions were significantly more abundant than Mb-type centric fusions at all irradiation doses. Dicentrics completely disappeared, but centric fusions and translocations were well transmitted from M0 to M1. Identification of specific chromosomes involved in some rearrangements was attempted by sequential fluorescence in situ hybridization with a mix of repeated DNA probes and GISH on the same slide. The irradiated amphiploids formed fewer seeds than untreated plants, but normal levels of fertility were recovered in their offspring. The irradiation-induced wheat - Ae. biuncialis intergenomic translocations will facilitate the successful introgression of drought tolerance and other alien traits into bread wheat.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0831-2796
pubmed:author
pubmed:issnType
Print
pubmed:volume
52
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
156-65
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Detection of intergenomic chromosome rearrangements in irradiated Triticum aestivum--Aegilops biuncialis amphiploids by multicolour genomic in situ hybridization.
pubmed:affiliation
Agricultural Research Institute of the Hungarian Academy of Sciences, H-2462, Martonvasar, POB 19, Hungary. molnarm@mail.mgki.hu
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't