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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2009-4-27
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pubmed:abstractText |
Renal reabsorption of inorganic phosphate (P(i)) is mainly mediated by the Na(+)-dependent P(i)-cotransporter NaPi-IIa that is expressed in the brush-border membrane (BBM) of renal proximal tubules. Regulation and apical expression of NaPi-IIa are known to depend on a network of interacting proteins. Most of the interacting partners identified so far associate with the COOH-terminal PDZ-binding motif (TRL) of NaPi-IIa. In this study GABA(A) receptor-associated protein (GABARAP) was identified as a novel interacting partner of NaPi-IIa applying a membrane yeast-two-hybrid system (MYTH 2.0) to screen a mouse kidney library with the TRL-truncated cotransporter as bait. GABARAP mRNA and protein are present in renal tubules, and the interaction of NaPi-IIa and GABARAP was confirmed by using glutathione S-transferase pulldowns from BBM and coimmunoprecipitations from transfected HEK293 cells. Amino acids 36-68 of GABARAP were identified as the determinant for the described interaction. The in vivo effects of this interaction were studied in a murine model. GABARAP(-/-) mice have reduced urinary excretion of P(i), higher Na(+)-dependent (32)P(i) uptake in BBM vesicles, and increased expression of NaPi-IIa in renal BBM compared with GABARAP(+/+) mice. The expression of Na(+)/H(+) exchanger regulatory factor (NHERF)1, an important scaffold for the apical expression of NaPi-IIa, is also increased in GABARAP(-/-) mice. The absence of GABARAP does not interfere with the regulation of the cotransporter by either parathyroid hormone or acute changes of dietary P(i) content.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-10742978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-10747018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-10899939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-10900017,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-10984509,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-11099500,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-11403569,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-11414770,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-11461150,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-11473652,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-11779480,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-11880379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-11997026,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-12075354,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-12169661,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-12192091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-12507496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-12952857,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-15325588,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-15530441,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-15601949,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-15695379,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-15932893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-16303767,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-16307606,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-16358214,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-16358215,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-16650615,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-16849419,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-16955105,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-16987995,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-17344426,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-17545973,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-18027972,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-18407958,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-18497328,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-18567048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-2535703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-3503531,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-6117319,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-8140421,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-9314537,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-9560157,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-9560251,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-9560283,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-9767538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19225049-9892355
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cytoskeletal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/GABARAP protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Parathyroid Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphorus, Dietary,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Slc34a1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Hydrogen Antiporter,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Phosphate Cotransporter...,
http://linkedlifedata.com/resource/pubmed/chemical/sodium-hydrogen exchanger...
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1931-857X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
296
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
F1118-28
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pubmed:dateRevised |
2011-4-28
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pubmed:meshHeading |
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