Linked Life Data

19221693

Source:http://linkedlifedata.com/resource/pubmed/id/19221693

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2009-3-5
pubmed:abstractText
The baculovirus expression vector system exploits the polyhedrin (polh) promoter for high expression of foreign proteins in insect cells. The mechanism of basal and hyperactivated transcription from this promoter, however, remains poorly understood. We have analyzed the 4-kb upstream region of the polh promoter; deletion of two separate parts of the 4-kb upstream region, harboring the Oct binding site and the heat shock element, respectively, resulted in significant reduction of reporter gene expression regulated by the polh promoter. Insect cell host factors could bind to these elements in vitro. Moreover, these elements could activate polh transcription during viral infection when present upstream of a minimal polh promoter in transient expression reporter assays. Our results suggest the possible existence of transcription factors belonging to the POU and heat shock transcription factor family in Spodoptera frugiperda cells and support the hypothesis that host proteins may play a major role in activating transcription from the polh promoter.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1432-8798
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
154
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
445-56
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Octamer and heat shock elements regulate transcription from the AcMNPV polyhedrin gene promoter.
pubmed:affiliation
Laboratory of Molecular Oncology, Centre for DNA Fingerprinting and Diagnostics, Block no. 7, 5-4-399/B, Gruhakalpa complex, Nampally, Hyderabad, 500001, India.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't