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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1977-5-20
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pubmed:abstractText |
Exposing micro-dissected pancreatic islets of non-inbred ob/ob mice to 2-5 mM-alloxan for 10 min decreased the ability of the islets to accumulate Rb+. Rb+ accumulation in pieces of exocrine pancreas was unaffected by alloxan. When islets were treated with alloxan in the presence of 2-20 mM-D-glucose, the Rb+-accumulating ability was protected in a dose-dependent manner. The protective action of D-glucose was reproduced with 3-O-methyl-D-glucose but not with L-glucose or D-mannoheptulose; mannoheptulose prevented D-glucose from exerting its protective action. The inhibition of Rb+ accumulation was due to a decreased inward pumping, since alloxan did not affect Rb+ efflux from pre-loaded islets. The inhibitory effect of alloxan had a latency of about 1 min, as revealed by experiments with dispersed islet cells in suspension. Alloxan-treated islets showed only a marginal decrease in ATP and no change in glucose 6-phosphate concentration. Although alloxan slightly decreased the hydrolysis of ATP in a subcellular fraction enriched in plasma membranes, this effect could not be attributed to a ouabain-sensitive adenosine triphosphatase. The plasma membranes exhibited a K+-activated hydrolysis of p-nitrophenyl phosphate; this enzyme activity too was insensitive to alloxan. Glucose may protect the univalent-cation pump by preventing permeation of alloxan via a path coupled to the hexose-transport system. Inhibition of the pump may be fundamental to the induction of alloxan-diabetes.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-1089908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-1092583,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-1103887,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-128691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-130057,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-13197372,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-13216176,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-14131777,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-322658,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-4198516,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-4199014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-4199636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-4261686,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-4266918,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-4288437,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-4375640,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-4378182,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-4442670,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-4469677,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-4551472,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-4569871,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-4574933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-4583060,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-4601168,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-4605120,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-4606675,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-4897289,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-4937446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-4942121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/192215-773724
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
162
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9-18
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:192215-Adenosine Triphosphate,
pubmed-meshheading:192215-Alloxan,
pubmed-meshheading:192215-Animals,
pubmed-meshheading:192215-Biological Transport, Active,
pubmed-meshheading:192215-Cell Membrane,
pubmed-meshheading:192215-Cell Survival,
pubmed-meshheading:192215-Glucose,
pubmed-meshheading:192215-Glucosephosphates,
pubmed-meshheading:192215-Islets of Langerhans,
pubmed-meshheading:192215-Mice,
pubmed-meshheading:192215-Phosphoric Monoester Hydrolases,
pubmed-meshheading:192215-Rubidium
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pubmed:year |
1977
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pubmed:articleTitle |
Alloxan cytotoxicity in vitro. Inhibition of rubidium ion pumping in pancreatic beta-cells.
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pubmed:publicationType |
Journal Article
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