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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2009-2-17
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pubmed:abstractText |
Bacterial DNA motifs (such as CpG-oligodeoxynucleotides: CpG-ODN) induce innate immune responses via binding to Toll-like-receptor-9 (TLR-9). In murine intestinal mucosa treatment with CpG-ODN worsens chronic intestinal inflammation, whereas it prevents or ameliorates colitis when given in a prophylactic setting. In tonsils B cells have been reported to express TLR-9, especially after activation. Whether B cells in the human intestinal mucosa also express TLR-9 and whether their function can be influenced by CpG-ODN is, so far, unknown. Mucosal B cells were isolated according to a new protocol from surgical specimens of patients with inflammatory bowel disease and from controls by collagenase digestion followed by magnetic cell sorting using anti-CD19 antibody armed magnetic beads. TLR-9 mRNA and protein expression were quantified by real-time polymerase chain reaction (PCR) and Western blot, respectively. Immunoglobulin A (IgA) secretion was measured by enzyme-linked immunosorbent assay after stimulation of isolated B cells with CpG-ODN, control GpC-ODN or lipopolysaccharide (LPS). Flow cytometric analysis of the isolated lamina propria mononuclear cells showed a purification of 73% (+/-22%) CD19(+) cells. By quantitative reverse transcription-PCR and by Western blot TLR-9 expression in this cell population was evident. IgA secretion was increased significantly by CpG-ODN incubation compared with GpC-ODN and LPS. Compared with unstimulated controls, CpG-ODN up-regulated IgA secretion to 139% (+/-21%). These data demonstrate that CD19(+) mucosal B cells express TLR-9 and secrete increased levels of IgA upon stimulation with CpG-ODN, indicating an additional link between adaptive and innate intestinal immune responses.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-11106939,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-11130078,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-11861616,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-11970999,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-11976718,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-11984528,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-12115616,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-12243247,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-12560217,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-12689944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-12778475,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-14616780,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-15383574,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-15383579,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-15532993,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-15653438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-15814663,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-15877894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-15879013,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-15886111,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-16048541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-17161619,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-17277148,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-17379700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-17485512,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-17570691,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-17621369,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-17906446,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-7700380,
http://linkedlifedata.com/resource/pubmed/commentcorrection/19220839-9467976
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD19,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD4,
http://linkedlifedata.com/resource/pubmed/chemical/CPG-oligonucleotide,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin A,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Toll-Like Receptor 9
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1365-2249
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
155
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
534-40
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pubmed:dateRevised |
2010-9-23
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pubmed:meshHeading |
pubmed-meshheading:19220839-Antigens, CD19,
pubmed-meshheading:19220839-Antigens, CD4,
pubmed-meshheading:19220839-B-Lymphocytes,
pubmed-meshheading:19220839-Blotting, Western,
pubmed-meshheading:19220839-Cell Separation,
pubmed-meshheading:19220839-Cells, Cultured,
pubmed-meshheading:19220839-Colon,
pubmed-meshheading:19220839-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:19220839-Flow Cytometry,
pubmed-meshheading:19220839-Humans,
pubmed-meshheading:19220839-Immunity, Innate,
pubmed-meshheading:19220839-Immunoglobulin A,
pubmed-meshheading:19220839-Intestinal Mucosa,
pubmed-meshheading:19220839-Lipopolysaccharides,
pubmed-meshheading:19220839-Oligodeoxyribonucleotides,
pubmed-meshheading:19220839-RNA, Messenger,
pubmed-meshheading:19220839-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:19220839-Stimulation, Chemical,
pubmed-meshheading:19220839-Toll-Like Receptor 9
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pubmed:year |
2009
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pubmed:articleTitle |
CpG-oligodeoxynucleotides stimulate immunoglobulin A secretion in intestinal mucosal B cells.
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pubmed:affiliation |
Department of Internal Medicine I, University of Regensburg, Regensburg, Germany. stefan.blaas@klinik.uni-regensburg.de
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pubmed:publicationType |
Journal Article
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