Linked Life Data

1922080

Source:http://linkedlifedata.com/resource/pubmed/id/1922080

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1991-10-29
pubmed:databankReference
pubmed:abstractText
Hypothalamic GRH gene expression has been shown to be negatively regulated by GH in both rat and mouse. The recent reports of different 5' untranslated sequences in mouse GRH cDNA from hypothalamus and placenta have raised the possibility of tissue-specific regulation of the GRH gene. To provide support for this possibility, we have studied rodent models with GH deficiency due to genetic defects in the pituitary. Complementary DNA probes for the hypothalamic and placental 5' regions were used to determine the tissue specificity of each mRNA. Although the hypothalamic form of GRH mRNA was detected in placenta, it constituted less than 0.7% of total placental GRH mRNA. A placental 5' probe (based on the previously reported sequence) hybridized only with a larger mRNA species and was not tissue specific, indicating that it was not related to GRH and was derived possibly from a cloning artifact. The correct 5' sequence of mouse placental GRH cDNA was determined and shown to be distinct from both that previously reported and the hypothalamic sequence. Although the placental form of GRH mRNA was detected in hypothalamus using the polymerase chain reaction, its levels were undetectable by Northern blotting. The 5' end of rat placental GRH cDNA was similarly sequenced and shown to exhibit no homology with the rat 5' hypothalamic sequence, but a high degree of homology with the corresponding mouse placental sequence. In GH-deficient dwarf (dw/dw) rats, hypothalamic GRH mRNA levels were significantly increased above control levels in both females and males, and pregnancy did not alter the levels in either (dw) or control rats.(ABSTRACT TRUNCATED AT 250 WORDS)
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0888-8809
pubmed:author
pubmed:issnType
Print
pubmed:volume
5
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
476-84
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:1922080-Animals, pubmed-meshheading:1922080-Base Sequence, pubmed-meshheading:1922080-Blotting, Northern, pubmed-meshheading:1922080-Blotting, Southern, pubmed-meshheading:1922080-Female, pubmed-meshheading:1922080-Gene Expression Regulation, pubmed-meshheading:1922080-Growth Hormone, pubmed-meshheading:1922080-Growth Hormone-Releasing Hormone, pubmed-meshheading:1922080-Hypothalamus, pubmed-meshheading:1922080-Liver, pubmed-meshheading:1922080-Male, pubmed-meshheading:1922080-Mice, pubmed-meshheading:1922080-Molecular Sequence Data, pubmed-meshheading:1922080-Organ Specificity, pubmed-meshheading:1922080-Placenta, pubmed-meshheading:1922080-Polymerase Chain Reaction, pubmed-meshheading:1922080-Pregnancy, pubmed-meshheading:1922080-RNA, Messenger, pubmed-meshheading:1922080-Rats, pubmed-meshheading:1922080-Rats, Inbred Lew, pubmed-meshheading:1922080-Sequence Homology, Nucleic Acid, pubmed-meshheading:1922080-Transcription, Genetic
pubmed:year
1991
pubmed:articleTitle
Tissue-specific transcription initiation and effects of growth hormone (GH) deficiency on the regulation of mouse and rat GH-releasing hormone gene in hypothalamus and placenta.
pubmed:affiliation
Department of Internal Medicine, University of Cincinnati College of Medicine, Ohio 45267.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.