Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
10
pubmed:dateCreated
1991-10-31
pubmed:abstractText
In the accompanying report (C. F. Webb, C. Das, S. Eaton, K. Calame, and P. Tucker, Mol. Cell. Biol. 11:5197-5205, 1991), we characterize B-cell-specific protein-DNA interactions at -500 and -200 bp upstream of the mu immunoglobulin heavy chain promoter whose abundances were increased by interleukin-5 plus antigen. Because of the high A + T/G + C ratio of these sequences and the consistent findings by others that enhancer- and promoterlike regions are often located near matrix-associated regions, we asked whether these sequences might also be involved in binding to the nuclear matrix. Indeed, DNA fragments containing the -500 binding site were bound by nuclear matrix proteins. Furthermore, UV cross-linking studies showed that the DNA binding site for interleukin-5-plus-antigen-inducible proteins could also bind to proteins solubilized from the nuclear matrix. Nuclear matrix-associated sequences have also been demonstrated on either side of the intronic immunoglobulin heavy chain enhancer. Our data suggest a topological model by which interactions among proteins bound to the promoter and distal enhancer sequences might occur.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/1922040-1697537, http://linkedlifedata.com/resource/pubmed/commentcorrection/1922040-1846084, http://linkedlifedata.com/resource/pubmed/commentcorrection/1922040-1922039, http://linkedlifedata.com/resource/pubmed/commentcorrection/1922040-2111008, http://linkedlifedata.com/resource/pubmed/commentcorrection/1922040-2114107, http://linkedlifedata.com/resource/pubmed/commentcorrection/1922040-2152827, http://linkedlifedata.com/resource/pubmed/commentcorrection/1922040-2480380, http://linkedlifedata.com/resource/pubmed/commentcorrection/1922040-2512290, http://linkedlifedata.com/resource/pubmed/commentcorrection/1922040-2546156, http://linkedlifedata.com/resource/pubmed/commentcorrection/1922040-2556260, http://linkedlifedata.com/resource/pubmed/commentcorrection/1922040-2655930, http://linkedlifedata.com/resource/pubmed/commentcorrection/1922040-2792763, http://linkedlifedata.com/resource/pubmed/commentcorrection/1922040-2797152, http://linkedlifedata.com/resource/pubmed/commentcorrection/1922040-2843296, http://linkedlifedata.com/resource/pubmed/commentcorrection/1922040-2922063, http://linkedlifedata.com/resource/pubmed/commentcorrection/1922040-3002631, http://linkedlifedata.com/resource/pubmed/commentcorrection/1922040-3015418, http://linkedlifedata.com/resource/pubmed/commentcorrection/1922040-3031052, http://linkedlifedata.com/resource/pubmed/commentcorrection/1922040-3039350, http://linkedlifedata.com/resource/pubmed/commentcorrection/1922040-3093895, http://linkedlifedata.com/resource/pubmed/commentcorrection/1922040-3118372, http://linkedlifedata.com/resource/pubmed/commentcorrection/1922040-3132558, http://linkedlifedata.com/resource/pubmed/commentcorrection/1922040-3277168, http://linkedlifedata.com/resource/pubmed/commentcorrection/1922040-3796614
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0270-7306
pubmed:author
pubmed:issnType
Print
pubmed:volume
11
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5206-11
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Identification of a matrix-associated region 5' of an immunoglobulin heavy chain variable region gene.
pubmed:affiliation
Department of Immunobiology, Oklahoma Medical Research Foundation, Oklahoma City 73104.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.