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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
2009-10-21
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pubmed:abstractText |
Signaling by Wnt/beta-catenin regulates self-renewal of tissue stem cells in the gut and, when activated in the embryonic bronchiolar epithelium, leads to stem cell expansion. We have used transgenic and cell type-specific knockout strategies to determine roles for beta-catenin-regulated gene expression in normal maintenance and repair of the bronchiolar epithelium. Analysis of TOPGal transgene activity detected beta-catenin signaling in the steady-state and repairing bronchiolar epithelium. However, the broad distribution and phenotype of signaling cells precluded establishment of a clear role for beta-catenin in the normal or repairing state. Necessity of beta-catenin signaling was tested through Cre-mediated deletion of Catnb exons 2-6 in airway epithelial cells. Functional knockout of beta-catenin had no impact on expression of Clara cell differentiation markers, mitotic index, or sensitivity of these cells to the Clara cell-specific toxicant, naphthalene. Repair of the naphthalene-injured airway proceeded with establishment of focal regions of beta-catenin-null epithelium. The size of regenerative epithelial units, mitotic index, and restoration of the ciliated cell population did not vary between wild-type and genetically modified mice. Thus, beta-catenin was not necessary for maintenance or efficient repair of the bronchiolar epithelium.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Catnb protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Cre recombinase,
http://linkedlifedata.com/resource/pubmed/chemical/Integrases,
http://linkedlifedata.com/resource/pubmed/chemical/Naphthalenes,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Scgb1a1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/TCF Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Tcf7l2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor 7-Like 2...,
http://linkedlifedata.com/resource/pubmed/chemical/Uteroglobin,
http://linkedlifedata.com/resource/pubmed/chemical/beta Catenin,
http://linkedlifedata.com/resource/pubmed/chemical/naphthalene
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1535-4989
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
41
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
535-43
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:19213872-Animals,
pubmed-meshheading:19213872-Bronchioles,
pubmed-meshheading:19213872-Cell Differentiation,
pubmed-meshheading:19213872-Cell Proliferation,
pubmed-meshheading:19213872-Gene Expression Regulation, Developmental,
pubmed-meshheading:19213872-Gene Knockout Techniques,
pubmed-meshheading:19213872-Integrases,
pubmed-meshheading:19213872-Lac Operon,
pubmed-meshheading:19213872-Mice,
pubmed-meshheading:19213872-Mice, Inbred C57BL,
pubmed-meshheading:19213872-Mice, Transgenic,
pubmed-meshheading:19213872-Mitotic Index,
pubmed-meshheading:19213872-Naphthalenes,
pubmed-meshheading:19213872-Phenotype,
pubmed-meshheading:19213872-Promoter Regions, Genetic,
pubmed-meshheading:19213872-RNA, Messenger,
pubmed-meshheading:19213872-Rats,
pubmed-meshheading:19213872-Regeneration,
pubmed-meshheading:19213872-Respiratory Mucosa,
pubmed-meshheading:19213872-Signal Transduction,
pubmed-meshheading:19213872-Stem Cells,
pubmed-meshheading:19213872-TCF Transcription Factors,
pubmed-meshheading:19213872-Time Factors,
pubmed-meshheading:19213872-Transcription Factor 7-Like 2 Protein,
pubmed-meshheading:19213872-Uteroglobin,
pubmed-meshheading:19213872-beta Catenin
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pubmed:year |
2009
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pubmed:articleTitle |
beta-Catenin is not necessary for maintenance or repair of the bronchiolar epithelium.
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pubmed:affiliation |
Center for Lung Regeneration, Department of Environmental and Occupational Health, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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