Linked Life Data

1921334

Source:http://linkedlifedata.com/resource/pubmed/id/1921334

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1991-11-7
pubmed:abstractText
Effects of chronic exposure to retinoic acid on a cell line (FRLE cells) established from the fetal rat lung type II alveolar epithelial cell have been studied. Chronic exposure to retinoic acid inhibited proliferation and altered the pattern of culture morphology at the light microscopic level. At the ultrastructural level, the development of lamellar body-like structures was inhibited. Although the rates of both total collagen and protein produced per cell were enhanced by chronic retinoic acid exposure, the increase in collagen production exceeded that of total protein synthesis. Chromatographic evaluation of collagen types I, III, IV, and V revealed increases in the amounts of radioactivity incorporated into each collagen type. However, the magnitudes of the increases differed for each collagen type, with the production of type IV collagen being the most enhanced. These analyses also indicated several additional effects on collagen production: 1) collagen type specific alterations in the ratio of secreted to cell associated molecules, 2) an increased ratio of type I homotrimers to type I heterotrimers, and 3) a decreased ratio of type V homotrimers to type V heterotrimers. These results indicate that chronic exposure to retinoic acid selectively affects collagen production in and differentiation of FRLE cells and establish that these changes are opposite to those observed under conditions of chronic exposure to EGF.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0023-6837
pubmed:author
pubmed:issnType
Print
pubmed:volume
65
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
441-50
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1991
pubmed:articleTitle
Extracellular matrix biosynthesis by cultured fetal rat lung epithelial cells. IV. Effects of chronic exposure to retinoic acid on growth, differentiation, and collagen biosynthesis.
pubmed:affiliation
Department of Pathology, Vanderbilt University School of Medicine, Nashville, Tennessee.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.