Linked Life Data

19211239

Source:http://linkedlifedata.com/resource/pubmed/id/19211239

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
2009-3-30
pubmed:abstractText
A simple approach that employs black drawing ink (BDI) as bulk fluorescence light blocker and improves considerably the homogeneous signal detection in capillary-waveguide fluoroimmunosensors is presented. The concept was proved using a capillary sensor configuration. Fluorescent molecules in the capillary were excited by a laser beam vertically to its axis and the emitted photons that were trapped and waveguided through the capillary wall were then collected. Two competitive fluoroimmunoassays, for rabbit gamma-globulins in buffer and thyroxine in human serum, respectively, were set-up to evaluate this approach. It was found that the presence of ink improved the specific to bulk fluorescence signal ratio by approximately 60-times without affecting the analyte-antibody binding reaction thus, facilitating homogeneous detection. The analytical characteristics of the two assays developed with the sensor operating in homogeneous detection mode were similar to those determined following the heterogeneous detection mode (i.e. after removal/washing of the immunoreaction mixture).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
1873-4235
pubmed:author
pubmed:issnType
Electronic
pubmed:day
15
pubmed:volume
24
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2735-9
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Bulk fluorescence light blockers to improve homogeneous detection in capillary-waveguide fluoroimmunosensors.
pubmed:affiliation
Institute of Radioisotopes & Radiodiagnostic Products, Immunoassay/Immunosensors Laboratory, NCSR Demokritos, Aghia Paraskevi, GR-15310, Greece.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies