| pubmed-article:19209351 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:19209351 | lifeskim:mentions | umls-concept:C0007634 | lld:lifeskim |
| pubmed-article:19209351 | lifeskim:mentions | umls-concept:C0439851 | lld:lifeskim |
| pubmed-article:19209351 | lifeskim:mentions | umls-concept:C0086296 | lld:lifeskim |
| pubmed-article:19209351 | lifeskim:mentions | umls-concept:C1704608 | lld:lifeskim |
| pubmed-article:19209351 | lifeskim:mentions | umls-concept:C1552596 | lld:lifeskim |
| pubmed-article:19209351 | lifeskim:mentions | umls-concept:C0439831 | lld:lifeskim |
| pubmed-article:19209351 | lifeskim:mentions | umls-concept:C1947931 | lld:lifeskim |
| pubmed-article:19209351 | pubmed:issue | 1 | lld:pubmed |
| pubmed-article:19209351 | pubmed:dateCreated | 2009-2-11 | lld:pubmed |
| pubmed-article:19209351 | pubmed:abstractText | The soft lithographic technique is a collection of simple and cost-effective patterning techniques which applies an elastomeric stamp to transfer a nano/micro-scale pattern. Patterning biological materials using soft lithography provides procedurally simple control of the surface chemistry and the cell environments. However, conventional methods for generating microstructures on a substrate require expensive clean room facilities and skillful training. Here we report a simple and inexpensive clean-room free process using a conventional photomask film as a master to fabricate elastomeric stamps or microfluidic channels. This ultra rapid prototyping technique was applied to print FITC labeled poly-L-lysine with a 10 microm feature size on a glass substrate using soft lithographic processes, such as micro-contact printing and micromolding in capillaries, for patterning human hepatocellular carcinoma cells, human skin fibroblasts and hippocampal neurons from E-18 Sprague-Dawley rat. This novel technique using a photomask film as a master would be very useful 'hands-on' tool for the generation of micro-patterned chemical or biological assays using cells and proteins. | lld:pubmed |
| pubmed-article:19209351 | pubmed:language | eng | lld:pubmed |
| pubmed-article:19209351 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19209351 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:19209351 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19209351 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:19209351 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:19209351 | pubmed:month | Jan | lld:pubmed |
| pubmed-article:19209351 | pubmed:issn | 1473-0197 | lld:pubmed |
| pubmed-article:19209351 | pubmed:author | pubmed-author:NamYoonkeyY | lld:pubmed |
| pubmed-article:19209351 | pubmed:author | pubmed-author:YeonJu HunJH | lld:pubmed |
| pubmed-article:19209351 | pubmed:author | pubmed-author:ParkJe-KyunJK | lld:pubmed |
| pubmed-article:19209351 | pubmed:author | pubmed-author:HwangHyundooH | lld:pubmed |
| pubmed-article:19209351 | pubmed:author | pubmed-author:KangGyuminG | lld:pubmed |
| pubmed-article:19209351 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:19209351 | pubmed:day | 7 | lld:pubmed |
| pubmed-article:19209351 | pubmed:volume | 9 | lld:pubmed |
| pubmed-article:19209351 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:19209351 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:19209351 | pubmed:pagination | 167-70 | lld:pubmed |
| pubmed-article:19209351 | pubmed:meshHeading | pubmed-meshheading:19209351... | lld:pubmed |
| pubmed-article:19209351 | pubmed:meshHeading | pubmed-meshheading:19209351... | lld:pubmed |
| pubmed-article:19209351 | pubmed:meshHeading | pubmed-meshheading:19209351... | lld:pubmed |
| pubmed-article:19209351 | pubmed:meshHeading | pubmed-meshheading:19209351... | lld:pubmed |
| pubmed-article:19209351 | pubmed:meshHeading | pubmed-meshheading:19209351... | lld:pubmed |
| pubmed-article:19209351 | pubmed:year | 2009 | lld:pubmed |
| pubmed-article:19209351 | pubmed:articleTitle | Direct rapid prototyping of PDMS from a photomask film for micropatterning of biomolecules and cells. | lld:pubmed |
| pubmed-article:19209351 | pubmed:affiliation | Department of Bio and Brain Engineering, KAIST, Yuseong-gu, Daejeon 305-701, Korea. | lld:pubmed |
| pubmed-article:19209351 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:19209351 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
