Linked Life Data

19209351

Source:http://linkedlifedata.com/resource/pubmed/id/19209351

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2009-2-11
pubmed:abstractText
The soft lithographic technique is a collection of simple and cost-effective patterning techniques which applies an elastomeric stamp to transfer a nano/micro-scale pattern. Patterning biological materials using soft lithography provides procedurally simple control of the surface chemistry and the cell environments. However, conventional methods for generating microstructures on a substrate require expensive clean room facilities and skillful training. Here we report a simple and inexpensive clean-room free process using a conventional photomask film as a master to fabricate elastomeric stamps or microfluidic channels. This ultra rapid prototyping technique was applied to print FITC labeled poly-L-lysine with a 10 microm feature size on a glass substrate using soft lithographic processes, such as micro-contact printing and micromolding in capillaries, for patterning human hepatocellular carcinoma cells, human skin fibroblasts and hippocampal neurons from E-18 Sprague-Dawley rat. This novel technique using a photomask film as a master would be very useful 'hands-on' tool for the generation of micro-patterned chemical or biological assays using cells and proteins.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
1473-0197
pubmed:author
pubmed:issnType
Print
pubmed:day
7
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
167-70
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Direct rapid prototyping of PDMS from a photomask film for micropatterning of biomolecules and cells.
pubmed:affiliation
Department of Bio and Brain Engineering, KAIST, Yuseong-gu, Daejeon 305-701, Korea.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't