Source:http://linkedlifedata.com/resource/pubmed/id/19206271
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
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| pubmed:dateCreated |
2009-2-11
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| pubmed:abstractText |
We report the synthesis and characterization of DNA-grafted poly(N-isopropylacrylamide) (PNIPAM) micelles, their assembly into multilayered thin films, and the subsequent generation and poly(ethylene glycol) (PEG) functionalization of DNA-PNIPAM microcapsules. Multilayer films were assembled by sequentially depositing DNA-grafted PNIPAM micelles containing the cDNA sequences polyA(30) or polyT(30) (i.e., PNIPAM-A(30) or PNIPAM-T(30)). DNA-polymer microcapsules were obtained by the alternate deposition of PNIPAM-A(30) and PNIPAM-T(30) onto silica particles, followed by removal of the template core. Upon removal of the silica core particle, shrinkage of between 30 and 50% was observed for the microcapsules. The presence of PNIPAM within the DNA-polymer hybrid film reduces the permeability of the microcapsules to macrosolutes (e.g., dextran) compared with microcapsules made solely of DNA. The hydrophobic core of the DNA-grafted PNIPAM micelles was designed to contain alkyne "click" groups, which were exploited to covalently couple azide-bearing low-fouling PEG to the DNA-PNIPAM microcapsules. The combination of hydrophobic and reactive "click" nanodomains, along with the degradability of DNA, offers a multifunctional and versatile DNA-polymer capsule system that is envisioned to find applications in the controlled delivery of therapeutics.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acrylic Resins,
http://linkedlifedata.com/resource/pubmed/chemical/Azides,
http://linkedlifedata.com/resource/pubmed/chemical/Capsules,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/Drug Carriers,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescein-5-isothiocyanate,
http://linkedlifedata.com/resource/pubmed/chemical/Micelles,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Polymers,
http://linkedlifedata.com/resource/pubmed/chemical/Quartz,
http://linkedlifedata.com/resource/pubmed/chemical/Silicon Dioxide,
http://linkedlifedata.com/resource/pubmed/chemical/Solvents,
http://linkedlifedata.com/resource/pubmed/chemical/poly-N-isopropylacrylamide
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| pubmed:status |
MEDLINE
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| pubmed:month |
Jan
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| pubmed:issn |
1936-086X
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| pubmed:author | |
| pubmed:issnType |
Electronic
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| pubmed:day |
27
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| pubmed:volume |
3
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| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
234-40
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| pubmed:meshHeading |
pubmed-meshheading:19206271-Acrylic Resins,
pubmed-meshheading:19206271-Azides,
pubmed-meshheading:19206271-Capsules,
pubmed-meshheading:19206271-Crystallization,
pubmed-meshheading:19206271-DNA,
pubmed-meshheading:19206271-Drug Carriers,
pubmed-meshheading:19206271-Fluorescein-5-isothiocyanate,
pubmed-meshheading:19206271-Micelles,
pubmed-meshheading:19206271-Microscopy, Confocal,
pubmed-meshheading:19206271-Oligonucleotides,
pubmed-meshheading:19206271-Polymers,
pubmed-meshheading:19206271-Quartz,
pubmed-meshheading:19206271-Silicon Dioxide,
pubmed-meshheading:19206271-Solvents,
pubmed-meshheading:19206271-Time Factors
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| pubmed:year |
2009
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| pubmed:articleTitle |
Assembly and functionalization of DNA-polymer microcapsules.
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| pubmed:affiliation |
Centre for Nanoscience and Nanotechnology, Department of Chemical and Biomolecular Engineering, The University of Melbourne, Parkville, Victoria 3010, Australia.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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