Linked Life Data

19205627

Source:http://linkedlifedata.com/resource/pubmed/id/19205627

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
2009-10-23
pubmed:abstractText
Cadmium, a toxic heavy metal, poses a significant threat to human health. Currently, the methods for detecting cadmium residue in farm produce need expensive equipment, intensive labor, and much time to finish one detection. In this study, a direct competitive enzyme-linked immunosorbent assay (DC-ELISA) based on a cadmium-chelate-specific monoclonal antibody has been developed. The DC-ELISA showed an IC(50) of 2.30 microg/L with a detection limit of 0.20 microg/L for cadmium. The assay has been demonstrated to be highly specific since the monoclonal antibody showed little or no cross-reactivity with all tested metal chelates which include Cd(2+), Pb(2+), Hg(2+), Zn(2+), Na(+), Ca(2+), Fe(3+), Mg(2+), Mn(2+), Cu(2+), Al(3+), Co(2+), Cr(2+), Ni(2+), Sn(2), and K(+). The assay showed that a mean recovery ranged from 100.47% to 103.86%, and the coefficients of variations for intra- and inter-assay were 1.73-7.14% and 3.63-6.81%, respectively. Then, several farm produces including wheat flour, apple juice, rice flour, and tea were analyzed for cadmium residue with DC-ELISA and graphite furnace atomic absorption spectroscopy (GFAAS). The correlation coefficient between the DC-ELISA and GFAAS was 0.99. It was demonstrated that the DC-ELISA can be used as a simple and economic method to detect and quantitate cadmium residue in farm produce.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1559-0291
pubmed:author
pubmed:issnType
Electronic
pubmed:volume
159
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
708-17
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Development of direct competitive enzyme-linked immunosorbent assay for the determination cadmium residue in farm produce.
pubmed:affiliation
School of Biosciences & Bioengineering, South China University of Technology, Guangzhou, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't