Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2009-2-10
pubmed:abstractText
Biogeochemical cyclic activity of the ars (arsenic resistance system) operon is arsB influx/efflux encoded by the ecological of Pseudomonas putida. This suggests that studying arsenite-oxidizing bacteria may lead to a better understanding of molecular geomicrobiology, which can be applied to the bioremediation of arsenic-contaminated mines. This is the first report in which multiple arsB-binding mechanisms have been used on indigenous bacteria. In ArsB (strains OS-5; ABB83931; OS-19; ABB04282 and RW-28; ABB88574), there are ten putative enzyme, Histidine (His) 131, His 133, His 137, Arginine (Arg) 135, Arg 137, Arg 161, Trptohan (Trp) 142, Trp 164, Trp 166, and Trp 171, which are each located in different regions of the partial sequence. The adenosine triphosphate (ATP)-binding cassette transports, binding affinities and associating ratable constants show that As-binding is comparatively insensitive to the location of the residues within the moderately stable alpha-helical structure. The alpha-helical structures in ArsB-permease and anion permease arsB have been shown to import/export arsenic in P. putida. We proposed that arsB residues, His 131, His 133, His 137, Arg 135, Arg 137, Arg 161, Trp 142, Trp 164, Trp 166, and Trp 171 are required for arsenic binding and activation of arsA/arsB or arsAB. This arsB influx/efflux pum-ping is important, and the effect in arsenic species change and mobility in mine soil has got a significantly ecological role because it allows arsenic oxidizing/reducing bacteria to control biogeochemical cycle of abandoned mines.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1001-0742
pubmed:author
pubmed:issnType
Print
pubmed:volume
20
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1348-55
pubmed:meshHeading
pubmed:year
2008
pubmed:articleTitle
Biogeochemical cyclic activity of bacterial arsB in arsenic-contaminated mines.
pubmed:affiliation
Department of Environment Science and Engineering, Gwangju Institute of Science and Technology (GIST), 261 Cheomdan-gwagiro, Buk-gu, Gwangju, Republic of Korea. jinsoosu@gist.ac.kr
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't