|
rdf:type |
|
|
lifeskim:mentions |
umls-concept:C0004611,
umls-concept:C0006104,
umls-concept:C0017262,
umls-concept:C0086418,
umls-concept:C0599691,
umls-concept:C0768762,
umls-concept:C1171362,
umls-concept:C1515670,
umls-concept:C1880022,
umls-concept:C1881878,
umls-concept:C1998793
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|
pubmed:issue |
2
|
|
pubmed:dateCreated |
2009-2-9
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|
pubmed:abstractText |
We have successfully expressed an active human brain serine racemase (hSR) with His-tag using moderate halophile. The purified His-hSR showed high elimination and racemization activities on L-serine: the elimination activity was 2.6-fold higher than racemization activity. Both enzyme activities showed an optimum reaction pH at around 9.0 and were stimulated 5- to 7-fold by such divalent cations as Mg++, Mn++ and Ca++.
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pubmed:language |
eng
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|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:issn |
0929-8665
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|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
16
|
|
pubmed:owner |
NLM
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|
pubmed:authorsComplete |
Y
|
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pubmed:pagination |
201-6
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pubmed:meshHeading |
pubmed-meshheading:19200045-Base Sequence,
pubmed-meshheading:19200045-Brain,
pubmed-meshheading:19200045-Cations, Divalent,
pubmed-meshheading:19200045-Chromatography, Affinity,
pubmed-meshheading:19200045-Cloning, Molecular,
pubmed-meshheading:19200045-Halomonas,
pubmed-meshheading:19200045-Humans,
pubmed-meshheading:19200045-Molecular Sequence Data,
pubmed-meshheading:19200045-Porins,
pubmed-meshheading:19200045-Promoter Regions, Genetic,
pubmed-meshheading:19200045-Protein Stability,
pubmed-meshheading:19200045-Racemases and Epimerases,
pubmed-meshheading:19200045-Serine
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|
pubmed:year |
2009
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|
pubmed:articleTitle |
Purification and characterization of human brain serine racemase expressed in moderately halophilic bacteria.
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|
pubmed:affiliation |
Applied and Molecular Microbiology, Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan. tokunaga@chem.agri.kagoshima-u.ac.jp
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pubmed:publicationType |
Journal Article
|
