Linked Life Data

19199604

Source:http://linkedlifedata.com/resource/pubmed/id/19199604

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2010-6-3
pubmed:abstractText
The synthesis of derivatives of metabolites from furazolidone, furaltadone, nitrofurazone, and nitrofurantoin using a new derivatizing reagent, 2-naphthaldehyde (NTA), is described. The reaction product was used in liquid chromatography with diode array detector (LC-DAD) for determination of protein-bound metabolites of nitrofurans in shrimp followed by two steps of liquid-liquid extraction. Derivatives of nitrofuran metabolites are well separated from NTA remaining in the extract upon separation on a ChromSpher 5 Pesticide (250 x 4.6 mm, 5 microm) column at 40 degrees C with acetonitrile/5 mM ammonium acetate adjusted to pH 7.5 gradient as the mobile phase and DAD detection at 308 nm except for naphthyl derivative of 1-aminohydantoin at 310 nm. The high absorptivity of these derivatives makes simultaneous screening of these metabolites in shrimp at 1 microg/kg possible for the first time using LC-DAD. The method was validated using blank shrimp fortified with all four metabolites at 1, 1.5, and 2 microg/kg. Recoveries were >86% with relative standard deviations of <14% for all four metabolites. Comparison between LC-DAD and APCI-MS/MS shows very good agreement for shrimp samples.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
1520-5118
pubmed:author
pubmed:issnType
Electronic
pubmed:day
11
pubmed:volume
57
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1752-9
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
New reagent for trace determination of protein-bound metabolites of nitrofurans in shrimp using liquid chromatography with diode array detector.
pubmed:affiliation
Department of Chemistry and Center for Innovation in Chemistry, Faculty of Science, Kasetsart University, Bangkok 10903, Thailand.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't, Evaluation Studies