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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
2009-2-27
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pubmed:abstractText |
Determining the long-range haplotypes in a diploid individual is a major technical challenge. Here we report a method of molecular haplotyping by directly imaging multiple polymorphic sites on individual human DNA molecules simultaneously. We demonstrate the utility of this technology by accurately determining the haplotypes consisting of up to 16 single-nucleotide polymorphisms in genomic regions up to 50 kilobases.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carbocyanines,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Single-Stranded,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Directed DNA Polymerase,
http://linkedlifedata.com/resource/pubmed/chemical/Exodeoxyribonucleases,
http://linkedlifedata.com/resource/pubmed/chemical/PLAT protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Tissue Plasminogen Activator,
http://linkedlifedata.com/resource/pubmed/chemical/cyanine dye 3,
http://linkedlifedata.com/resource/pubmed/chemical/cyanine dye 5
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1548-7105
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:volume |
6
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
199-201
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pubmed:meshHeading |
pubmed-meshheading:19198595-Carbocyanines,
pubmed-meshheading:19198595-Chromosomes, Human, Pair 17,
pubmed-meshheading:19198595-Chromosomes, Human, Pair 7,
pubmed-meshheading:19198595-Chromosomes, Human, Pair 8,
pubmed-meshheading:19198595-DNA,
pubmed-meshheading:19198595-DNA, Single-Stranded,
pubmed-meshheading:19198595-DNA Primers,
pubmed-meshheading:19198595-DNA-Directed DNA Polymerase,
pubmed-meshheading:19198595-Exodeoxyribonucleases,
pubmed-meshheading:19198595-Genetic Techniques,
pubmed-meshheading:19198595-Haplotypes,
pubmed-meshheading:19198595-Heterozygote,
pubmed-meshheading:19198595-Homozygote,
pubmed-meshheading:19198595-Humans,
pubmed-meshheading:19198595-Microscopy, Fluorescence,
pubmed-meshheading:19198595-Polymerase Chain Reaction,
pubmed-meshheading:19198595-Polymorphism, Single Nucleotide,
pubmed-meshheading:19198595-Sequence Analysis, DNA,
pubmed-meshheading:19198595-Staining and Labeling,
pubmed-meshheading:19198595-Tissue Plasminogen Activator
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pubmed:year |
2009
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pubmed:articleTitle |
Direct determination of haplotypes from single DNA molecules.
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pubmed:affiliation |
Cardiovascular Research Institute, University of California-San Francisco, 513 Parnassus Ave., San Francisco, CA 94143, USA. mingx@bionanomatrix.com
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pubmed:publicationType |
Journal Article,
Research Support, N.I.H., Extramural
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