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pubmed-article:1919505pubmed:abstractTextRhodococcus rhodochrous ATCC 4275 (Nocardia corallina) has a restriction-modification system with the same recognition sequence, methylation site and cleavage site as the SalI restriction-modification system. Both the restriction endonuclease and the DNA-methyltransferase (DNA-MTase) have been partially purified and characterized. The nuclease has requirements of activity similar to SalI, and a native Mr of about 46,000. The DNA-MTase is a protein with an Mr of about 67,000. No DNA homology was detected between the cloned salI restriction-modification genes of Streptomyces albus and R. rhodochrous chromosomal DNA.lld:pubmed
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pubmed-article:1919505pubmed:dateRevised2008-8-21lld:pubmed
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pubmed-article:1919505pubmed:articleTitleCharacterization of Rrh4273I, a restriction-modification system of Rhodococcus rhodochrous ATCC 4273 (Nocardia corallina) which recognizes the same sequence as the Streptomyces albus G SalI restriction-modification system.lld:pubmed
pubmed-article:1919505pubmed:affiliationArea de Microbiologia, Departamento de Biologia Funcional, Facultad de Medicina, Universidad de Oviedo, Spain.lld:pubmed
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