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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
6
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pubmed:dateCreated |
1991-11-8
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pubmed:abstractText |
Rhodococcus rhodochrous ATCC 4275 (Nocardia corallina) has a restriction-modification system with the same recognition sequence, methylation site and cleavage site as the SalI restriction-modification system. Both the restriction endonuclease and the DNA-methyltransferase (DNA-MTase) have been partially purified and characterized. The nuclease has requirements of activity similar to SalI, and a native Mr of about 46,000. The DNA-MTase is a protein with an Mr of about 67,000. No DNA homology was detected between the cloned salI restriction-modification genes of Streptomyces albus and R. rhodochrous chromosomal DNA.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Bacterial,
http://linkedlifedata.com/resource/pubmed/chemical/DNA modification methylase SalGI,
http://linkedlifedata.com/resource/pubmed/chemical/Deoxyribonucleases, Type II...,
http://linkedlifedata.com/resource/pubmed/chemical/GTCGAC-specific type II...,
http://linkedlifedata.com/resource/pubmed/chemical/Site-Specific...
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
0022-1287
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
137
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1279-84
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pubmed:dateRevised |
2008-8-21
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pubmed:meshHeading |
pubmed-meshheading:1919505-Base Sequence,
pubmed-meshheading:1919505-DNA, Bacterial,
pubmed-meshheading:1919505-Deoxyribonucleases, Type II Site-Specific,
pubmed-meshheading:1919505-Methylation,
pubmed-meshheading:1919505-Molecular Sequence Data,
pubmed-meshheading:1919505-Molecular Weight,
pubmed-meshheading:1919505-Rhodococcus,
pubmed-meshheading:1919505-Sequence Homology, Nucleic Acid,
pubmed-meshheading:1919505-Site-Specific DNA-Methyltransferase (Adenine-Specific)
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pubmed:year |
1991
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pubmed:articleTitle |
Characterization of Rrh4273I, a restriction-modification system of Rhodococcus rhodochrous ATCC 4273 (Nocardia corallina) which recognizes the same sequence as the Streptomyces albus G SalI restriction-modification system.
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pubmed:affiliation |
Area de Microbiologia, Departamento de Biologia Funcional, Facultad de Medicina, Universidad de Oviedo, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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