pubmed-article:19193635 | rdf:type | pubmed:Citation | lld:pubmed |
pubmed-article:19193635 | lifeskim:mentions | umls-concept:C0144555 | lld:lifeskim |
pubmed-article:19193635 | lifeskim:mentions | umls-concept:C0035679 | lld:lifeskim |
pubmed-article:19193635 | lifeskim:mentions | umls-concept:C0084829 | lld:lifeskim |
pubmed-article:19193635 | lifeskim:mentions | umls-concept:C0040649 | lld:lifeskim |
pubmed-article:19193635 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
pubmed-article:19193635 | lifeskim:mentions | umls-concept:C1519595 | lld:lifeskim |
pubmed-article:19193635 | pubmed:issue | 14 | lld:pubmed |
pubmed-article:19193635 | pubmed:dateCreated | 2009-3-30 | lld:pubmed |
pubmed-article:19193635 | pubmed:abstractText | To better understand the mechanism of steps in early transcription by RNA polymerase II (pol II), we investigated the molecular determinants of transcript slipping within complexes assembled on promoters containing a pre-melted transcription bubble from -9 to +3. Transcript slippage occurs when an RNA transcript contains a repetitive sequence that allows the transcript to slip back and pair with the template strand of the DNA at a new register before transcription continues. We established the contributions of individual transcription factors, DNA elements, and RNA length to slipping on a heteroduplex template using a highly purified human pol II transcription system. We found that transcripts slip at a very defined point in the transcription reaction, after pol II completes phosphodiester bond synthesis at register +5. This point is set by the position of the polymerase active site on the DNA template, as opposed to the length of the transcript, as well as by a repetitive CUCU sequence that must occur from +2 to +5. Interestingly, slipping at this juncture is induced by TATA-binding protein and transcription factor IIB and requires a TATA box but not a transcription factor IIB recognition sequence. We propose a model in which transcribing complexes, upon completing phosphodiester bond synthesis at register +5, enter one of two branches in which they either complete productive synthesis of the transcript or undergo multiple rounds of transcript slipping. | lld:pubmed |
pubmed-article:19193635 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:commentsCorrections | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:language | eng | lld:pubmed |
pubmed-article:19193635 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:citationSubset | IM | lld:pubmed |
pubmed-article:19193635 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
pubmed-article:19193635 | pubmed:status | MEDLINE | lld:pubmed |
pubmed-article:19193635 | pubmed:month | Apr | lld:pubmed |
pubmed-article:19193635 | pubmed:issn | 0021-9258 | lld:pubmed |
pubmed-article:19193635 | pubmed:author | pubmed-author:KugelJennifer... | lld:pubmed |
pubmed-article:19193635 | pubmed:author | pubmed-author:GoodrichJames... | lld:pubmed |
pubmed-article:19193635 | pubmed:author | pubmed-author:DrullingerLin... | lld:pubmed |
pubmed-article:19193635 | pubmed:author | pubmed-author:GilmanBenjami... | lld:pubmed |
pubmed-article:19193635 | pubmed:issnType | Print | lld:pubmed |
pubmed-article:19193635 | pubmed:day | 3 | lld:pubmed |
pubmed-article:19193635 | pubmed:volume | 284 | lld:pubmed |
pubmed-article:19193635 | pubmed:owner | NLM | lld:pubmed |
pubmed-article:19193635 | pubmed:authorsComplete | Y | lld:pubmed |
pubmed-article:19193635 | pubmed:pagination | 9093-8 | lld:pubmed |
pubmed-article:19193635 | pubmed:dateRevised | 2010-9-23 | lld:pubmed |
pubmed-article:19193635 | pubmed:meshHeading | pubmed-meshheading:19193635... | lld:pubmed |
pubmed-article:19193635 | pubmed:meshHeading | pubmed-meshheading:19193635... | lld:pubmed |
pubmed-article:19193635 | pubmed:meshHeading | pubmed-meshheading:19193635... | lld:pubmed |
pubmed-article:19193635 | pubmed:meshHeading | pubmed-meshheading:19193635... | lld:pubmed |
pubmed-article:19193635 | pubmed:meshHeading | pubmed-meshheading:19193635... | lld:pubmed |
pubmed-article:19193635 | pubmed:meshHeading | pubmed-meshheading:19193635... | lld:pubmed |
pubmed-article:19193635 | pubmed:meshHeading | pubmed-meshheading:19193635... | lld:pubmed |
pubmed-article:19193635 | pubmed:meshHeading | pubmed-meshheading:19193635... | lld:pubmed |
pubmed-article:19193635 | pubmed:meshHeading | pubmed-meshheading:19193635... | lld:pubmed |
pubmed-article:19193635 | pubmed:meshHeading | pubmed-meshheading:19193635... | lld:pubmed |
pubmed-article:19193635 | pubmed:meshHeading | pubmed-meshheading:19193635... | lld:pubmed |
pubmed-article:19193635 | pubmed:meshHeading | pubmed-meshheading:19193635... | lld:pubmed |
pubmed-article:19193635 | pubmed:year | 2009 | lld:pubmed |
pubmed-article:19193635 | pubmed:articleTitle | TATA-binding protein and transcription factor IIB induce transcript slipping during early transcription by RNA polymerase II. | lld:pubmed |
pubmed-article:19193635 | pubmed:affiliation | Department of Chemistry, University of Colorado, Boulder, Colorado 80309-0215, USA. | lld:pubmed |
pubmed-article:19193635 | pubmed:publicationType | Journal Article | lld:pubmed |
pubmed-article:19193635 | pubmed:publicationType | Research Support, U.S. Gov't, Non-P.H.S. | lld:pubmed |
entrez-gene:2959 | entrezgene:pubmed | pubmed-article:19193635 | lld:entrezgene |
http://linkedlifedata.com/r... | entrezgene:pubmed | pubmed-article:19193635 | lld:entrezgene |