Statements in which the resource exists.
SubjectPredicateObjectContext
pubmed-article:19193635rdf:typepubmed:Citationlld:pubmed
pubmed-article:19193635lifeskim:mentionsumls-concept:C0144555lld:lifeskim
pubmed-article:19193635lifeskim:mentionsumls-concept:C0035679lld:lifeskim
pubmed-article:19193635lifeskim:mentionsumls-concept:C0084829lld:lifeskim
pubmed-article:19193635lifeskim:mentionsumls-concept:C0040649lld:lifeskim
pubmed-article:19193635lifeskim:mentionsumls-concept:C0205263lld:lifeskim
pubmed-article:19193635lifeskim:mentionsumls-concept:C1519595lld:lifeskim
pubmed-article:19193635pubmed:issue14lld:pubmed
pubmed-article:19193635pubmed:dateCreated2009-3-30lld:pubmed
pubmed-article:19193635pubmed:abstractTextTo better understand the mechanism of steps in early transcription by RNA polymerase II (pol II), we investigated the molecular determinants of transcript slipping within complexes assembled on promoters containing a pre-melted transcription bubble from -9 to +3. Transcript slippage occurs when an RNA transcript contains a repetitive sequence that allows the transcript to slip back and pair with the template strand of the DNA at a new register before transcription continues. We established the contributions of individual transcription factors, DNA elements, and RNA length to slipping on a heteroduplex template using a highly purified human pol II transcription system. We found that transcripts slip at a very defined point in the transcription reaction, after pol II completes phosphodiester bond synthesis at register +5. This point is set by the position of the polymerase active site on the DNA template, as opposed to the length of the transcript, as well as by a repetitive CUCU sequence that must occur from +2 to +5. Interestingly, slipping at this juncture is induced by TATA-binding protein and transcription factor IIB and requires a TATA box but not a transcription factor IIB recognition sequence. We propose a model in which transcribing complexes, upon completing phosphodiester bond synthesis at register +5, enter one of two branches in which they either complete productive synthesis of the transcript or undergo multiple rounds of transcript slipping.lld:pubmed
pubmed-article:19193635pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:commentsCorrectionshttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:languageenglld:pubmed
pubmed-article:19193635pubmed:journalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:citationSubsetIMlld:pubmed
pubmed-article:19193635pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:chemicalhttp://linkedlifedata.com/r...lld:pubmed
pubmed-article:19193635pubmed:statusMEDLINElld:pubmed
pubmed-article:19193635pubmed:monthAprlld:pubmed
pubmed-article:19193635pubmed:issn0021-9258lld:pubmed
pubmed-article:19193635pubmed:authorpubmed-author:KugelJennifer...lld:pubmed
pubmed-article:19193635pubmed:authorpubmed-author:GoodrichJames...lld:pubmed
pubmed-article:19193635pubmed:authorpubmed-author:DrullingerLin...lld:pubmed
pubmed-article:19193635pubmed:authorpubmed-author:GilmanBenjami...lld:pubmed
pubmed-article:19193635pubmed:issnTypePrintlld:pubmed
pubmed-article:19193635pubmed:day3lld:pubmed
pubmed-article:19193635pubmed:volume284lld:pubmed
pubmed-article:19193635pubmed:ownerNLMlld:pubmed
pubmed-article:19193635pubmed:authorsCompleteYlld:pubmed
pubmed-article:19193635pubmed:pagination9093-8lld:pubmed
pubmed-article:19193635pubmed:dateRevised2010-9-23lld:pubmed
pubmed-article:19193635pubmed:meshHeadingpubmed-meshheading:19193635...lld:pubmed
pubmed-article:19193635pubmed:meshHeadingpubmed-meshheading:19193635...lld:pubmed
pubmed-article:19193635pubmed:meshHeadingpubmed-meshheading:19193635...lld:pubmed
pubmed-article:19193635pubmed:meshHeadingpubmed-meshheading:19193635...lld:pubmed
pubmed-article:19193635pubmed:meshHeadingpubmed-meshheading:19193635...lld:pubmed
pubmed-article:19193635pubmed:meshHeadingpubmed-meshheading:19193635...lld:pubmed
pubmed-article:19193635pubmed:meshHeadingpubmed-meshheading:19193635...lld:pubmed
pubmed-article:19193635pubmed:meshHeadingpubmed-meshheading:19193635...lld:pubmed
pubmed-article:19193635pubmed:meshHeadingpubmed-meshheading:19193635...lld:pubmed
pubmed-article:19193635pubmed:meshHeadingpubmed-meshheading:19193635...lld:pubmed
pubmed-article:19193635pubmed:meshHeadingpubmed-meshheading:19193635...lld:pubmed
pubmed-article:19193635pubmed:meshHeadingpubmed-meshheading:19193635...lld:pubmed
pubmed-article:19193635pubmed:year2009lld:pubmed
pubmed-article:19193635pubmed:articleTitleTATA-binding protein and transcription factor IIB induce transcript slipping during early transcription by RNA polymerase II.lld:pubmed
pubmed-article:19193635pubmed:affiliationDepartment of Chemistry, University of Colorado, Boulder, Colorado 80309-0215, USA.lld:pubmed
pubmed-article:19193635pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:19193635pubmed:publicationTypeResearch Support, U.S. Gov't, Non-P.H.S.lld:pubmed
entrez-gene:2959entrezgene:pubmedpubmed-article:19193635lld:entrezgene
http://linkedlifedata.com/r...entrezgene:pubmedpubmed-article:19193635lld:entrezgene