Linked Life Data

19189201

Source:http://linkedlifedata.com/resource/pubmed/id/19189201

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
2009-5-20
pubmed:abstractText
To enhance the role targeting, design to link NGR sequence with tumstatin active peptides-T(7)'s C-terminal, the derivant called T(7)-NGR. The cloning vector pMD-T(7) and pMD-T(7) N were constructed by PCR and gene synthesis methods, respectively, identified by digestion and DNA sequencing. After the digested plasmids were isolated by the low melting point agarose electrophoresis, the target-fragment was cut off and mixed with the recovery of the digested vector pET28a. Expression vector pET-T(7) and pET-T(7) N were constructed in low melting point agarose, identified by digestion and DNA sequencing, transformed into competent Escherichia coli BL21 (DE3), induced by IPTG. Identification result shows that pET-T(7) and pET-T(7) N were correct. Tricine-SDS-PAGE results showed that IPTG concentration of 1 mM, after the induction of 25 degrees C, 8 h, T(7) peptides and T(7)-NGR peptides have achieved the optimum conditions of expression. In conclusion, the expression vectors of the two peptides has been successfully constructed, and got product, no coverage at home and abroad, laid the foundation for further activity experiments.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
1591-8890
pubmed:author
pubmed:issnType
Print
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
165-71
pubmed:meshHeading
pubmed:year
2009
pubmed:articleTitle
Cloning and expression of the tumstatin active peptides-T(7) and its derivant-T(7)-NGR.
pubmed:affiliation
Key Lab of Tianjin Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, 300192 Tianjin, China.
pubmed:publicationType
Journal Article